Application: EX-CELL™ 325 PF CHO is a protein-free medium which has been developed for the growth of Chinese Hamster Ovary (CHO) cells. Because it contains no large macromolecules, EX-CELL 325 PF CHO facilitates the isolation and purification of secreted proteins from the cells. CHO cells propagated in EX-CELL 302 serum-free media can be transferred directly into this protein-free medium without extensive weaning protocols. This medium is supplied without L-glutamine and does not contain purines or pyrimidines to provide an appropriate medium for specialized CHO cell lines (i.e., Glutamine Synthetase, or the GS System™, and DHFR- selection systems).
Legal Information: EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC
Storage: 2-8°C; 2-8°C
Quantity: Formulated to contain 21.0 grams of powder per liter of medium.
Reconstitution: Aseptically add 20-40 ml of 200 mM L-glutamine solution per liter of medium prior to use.
Empirical Formula (Hill Notation): C26H48P2