Chromatography Columns

Chromatography Columns are essential components in separating chemical mixtures, enabling the analysis and purification of substances.

These columns, including poly prep chromatography columns, come in various formats to accommodate different chromatographic techniques, such as liquid chromatography (LC) and high-performance liquid chromatography (HPLC). The choice of column material and size directly influences the efficiency of separation, highlighting the importance of selecting the appropriate column for specific applications.

Chromatography columns facilitate the detailed analysis of complex samples, contributing significantly to advancements in pharmaceuticals, environmental science, and biotechnology. Their role in identifying, quantifying, and purifying compounds makes them a key to modern analytical laboratories.

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  • The ZORBAX column family is one of the most popular high-performance liquid chromatography (HPLC) column families for reversed-phase HPLC. ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution. From Eclipse Plus, Eclipse XDB,…

  • Bond Elut C18 is the most hydrophobic, bonded silica sorbent in the Bond Elut range. It is the most popular SPE sorbent because of its retentive nature for nonpolar compounds. C18 is generally regarded as having the broadest spectrum of retention among bonded silica sorbents, since it retains most…

  • Agilent Technologies

    Agilent Technologies products are intended for sale/shipment within the US only. International customers should contact their local Agilent Technologies distributor to place orders for Agilent Technologies products.

  • InfinityLab Poroshell 120 HILIC columns for hydrophilic interaction liquid chromatography (HILIC) are designed for the retention and high-resolution separation of small polar analytes. The superficially porous character of the particles decreases equilibration times to improve throughput for both…

  • YMC-Pack TMS columns are bonded with trimethylmonochlorosilane to create a phase with intermediate polarity for analysis of extremely hydrophobic compounds using conventional reversed-phase solvents and highly polar compounds using highly aqueous solvents. The unique chemistry of TMS is also…

  • Purospher® STAR RP-18 endcapped is the best choice for HPLC separation of basic, neutral, metal chelating samples or indeed any other format. Its outstanding performance and stability, allows a maximum flexibility in method development. Robust methods can be developed across the entire pH…

  • Agilent Technologies

    PL-SCX strong cation-exchange materials are extremely hydrophilic and are designed for purification of biomolecules. The materials are totally polymeric and are chemically and thermally stable over a full range of HPLC conditions. The strong cation-exchange functionalities, covalently linked to a…

  • Agilent Technologies

    Agilent J&W DB-1 is nonpolar and low-bleed, and operates under high temperature limits. It is an excellent choice for general purpose use in a wide variety of applications. Precision-engineered DB-1 is bonded, crosslinked and solvent rinsable, and is equivalent to USP phase G2. This product is…

  • Agilent Technologies

    Bond Elut C18 OH cartridge, tabless, 500 mg, 6 mL, 40 µm, 96/pk. A nonendcapped version of C18 that enables the silanols on the silica surface to be more active. A good choice for the fractionation of metabolites and enhanced retention of basic compounds. This product is only available to U.S.…

  • The Agilent Pursuit family of HPLC columns offers reliable performance in various common particle sizes and phase chemistries, including USP L1, USP L7, and USP L11. Pursuit columns are available in many dimensions, including preparative HPLC columns. In addition, both traditional (Pursuit) and…

  • Agilent Technologies

    Agilent J&W DB-17 is a (50%-phenyl)-methylpolysiloxane column of mid polarity. Equivalent to USP phase G3, it is excellent for confirmation analysis. This product is only available to U.S. Domestic Customers. For research use only, not to be used in diagnostic procedures.

  • The Bond Elut Certify extraction cartridge is a mixed mode sorbent containing nonpolar and C8 strong cation exchanger functionalities. Certify is most commonly used to extract basic (cationic) drugs from urine and blood, but is also effective for the extraction of many compounds from a diverse…

  • Agilent Technologies

    Agilent J&W HP-PLOT Al2O3 S is the standard column choice for light hydrocarbon analysis, particularly C1 to C8 hydrocarbon isomers. A mid-polar range alumina phase deactivated with sodium sulfate, it is excellent for resolving acetylene from butylene and isobutane. This product is only available…

  • YMC-Pack Polyamine II is a silica-based packing bonded with polyamine. It is particularly useful for separation of sugars. The column life of YMC-Pack Polyamine II in aqueous mobile phase is longer than conventional silica-based amino columns, and thus is applicable to separation of…

  • MilliporeSigma

    Dispersive SPE (dSPE), often referred to as the "QuEChERS" method (Quick, Easy, Cheap, Effective, Rugged, and Safe), is modern sample prep technique that is becoming increasingly popular in the area of multi-residue pesticide analysis in food and agricultural products. Using the QuEChERS method,…

  • LiChrospher 100 RP-18, 5 µm, 3.0 x 125 mm cartridge (AC) (use with hardware kit 5021-1845) Agilent Technologies products are intended for sale/shipment within the US only. International customers should contact their local Agilent Technologies distributor to place orders for Agilent…

  • AdvanceBio Peptide Mapping columns are designed to provide high-resolution peptide maps for protein identification and determination of post translation modifications. These reversed-phase HPLC columns provide the ability for you to quickly resolve and identify amino acid…

  • Agilent Technologies

    Bond Elut 96 square-well plates facilitate transfer from cartridge-based methods to 96-well plate automated formats. The 2 mL wells accommodate the larger processing volumes from older methods, easing method transfer and revalidation. When larger samples or wash volumes are required, the 2 mL well…

  • PLRP-S for Biomolecules products are available in a range of pore sizes and particle sizes, all with identical chemistry and fundamental adsorptive characteristics. The particles are inherently hydrophobic so there is no bonded phase, alkyl ligand required for reversed-phase separations. This gives…

  • Agilent Technologies

    Eclipse Plus C18 columns are designed for superior peak shape with basic compounds and deliver high efficiency and excellent peak shape with all sample types. Eclipse Plus C18 is especially useful for the separation of acidic, basic, and other highly polar compounds by reverse-phase liquid…

  • The Agilent Pursuit family of HPLC columns offers reliable performance in various common particle sizes and phase chemistries, including USP L1, USP L7, and USP L11. Pursuit columns are available in many dimensions, including preparative HPLC columns. In addition, both traditional (Pursuit) and…

  • Agilent Technologies

    Agilent Technologies products are intended for sale/shipment within the US only. International customers should contact their local Agilent Technologies distributor to place orders for Agilent Technologies products.

  • J&W Packed Gas Chromatography (GC) Columns are designed and manufactured to offer excellent and reproducible performance for all sample types associated with packed column separations, most important in the hydrocarbon processing industry. The efficient and rigorous packing technology used in J&W…

  • AttractSPE are based upon polymeric sorbents dedicated to sample cleanup for the extraction of compounds from complex matrices. AttractSPE combine the advantages of robustness, simplified method development, wide applicability and are not affected by drying out. The combination of the…

  • Agilent Technologies

    Agilent J&W CP-Sil 8 CB is a general-purpose phase that incorporates 5% phenyl groups in the dimethylpolysiloxane polymer, giving it slightly higher polarity than CP-Sil 5 CB. This delivers better selectivity for aromatic compounds and makes it the best choice for method development. Equivalent to…

  • LiChrospher® RP-18 is a reliable and versatile traditionally produced spherical silica gel carrier with C-18 reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds. This sorbent is packed into a LiChroCART® 250-4 HPLC cartridge. The…

  • Agilent Technologies

    100% Dimethylpolysiloxane Non-polar Specially processed for extended temperature limit of 400°C High temperature, polyimide-coated, fused silica tubing Excellent peak shape and faster elution times for high boilers Bonded and cross-linked Solvent rinsable …

  • Agilent Technologies

    Gas Chromatography (GC) columns are at the heart of the gas chromatograph and responsible for the separation process. GC is one of the most important and critical tools in analytical chemistry, because of its effectiveness, sensitivity and ease-of-use. GC columns contain a stationary phase,…

  • Agilent Technologies

    (35%-Phenyl)-methylpolysiloxane Midpolarity - slightly more polar than HP-35 Low bleed Inert to active solutes Ideal for confirmational analyses Bonded and cross-linked Solvent rinsable Equivalent to USP Phase G42 Similar Phases: Rtx-35, SPB-35, AT-35,…

  • Agilent Technologies

    Agilent J&W DB-WAX is ideal for food, fragrance, and flavor applications. The column's lower temperature limit of 20 °C improves resolution of analytes with low boiling points. Agilent offers a full range of PEG columns. Even though each phase is based on the polyethylene glycol polymer, strict…

  • A medium polarity sorbent with many uses, Bond Elut Cyano (CN) SPE products are available as either endcapped (CN-E) or unendcapped (CN-U) versions. Both the Bond Elut CN-E and Bond Elut CN-U products are available in a 40 µm particle size. Bond Elut CN-E is ideal for applications in which…

  • YMC Carotenoid is a silica-based reversed-phase column chemically bonded with a triacontyl (C30) group, and is useful for separating geometric isomers of carotenoids. It is useful as a column with different separation selectivity when it is difficult to separate compounds other than carotenoids…

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