Synonyms: 2′-Deoxyuridine 5′-triphosphate sodium salt solution; dUTP sodium salt
Empirical Formula (Hill Notation): C9H15N2O14P3 ¬? xNa+
Formula Weight: 468.14 (free acid basis)
Linear Formula: C9H15N2O14P3 ¬? xNa+
MDL Number: MFCD01630759
Purity: <0.9% (dNDP); 99% (dNTP)
Storage: −20°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
dUTP, PCR Grade, is suitable for many applications where high-quality reagents are required. Avoid carryover contamination between PCRs to eliminate a source of false positives.
General description:
dUTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3).
Other Notes:
PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).
Quality:
Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.
Physical form:
solution of dUTP water (pH 8.3).
Preparation Note:
Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP (10 mM, each), dUTP (30 mM); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP (each) and 30 μl dUTP to 40 μl Water,PCR grade).