Purity: ‚≥85% (HPLC)
Storage: −20°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
Digoxigenin-11-UTP has been used for labeling RNA probes for in situ hybridization.
Biochem/physiol Actions:
Linearized template DNA with T7, SP6, or T3 promoter can be in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids.
General description:
Digoxigenin-11-UTP is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for DIG (digoxigenin) labeling of RNA in a ratio of 35:65%.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Typical analysis: 85% DIG-11-UTP (HPLC, area%)
Function tested with the DIG RNA Labeling Kit.
Formula: C43H61N4O22P3Li4