Optimizing Efficiency Within the PCR Workflow

scientist working in PCR lab

Discussing the importance of maintaining an efficient, error-free workflow when performing PCR testing.

The PCR workflow is a critical part of many scientific and laboratory procedures. In order to ensure accurate results, it is essential to maintain a high level of efficiency throughout the process. There are several key steps that can be taken to optimize efficiency within the PCR workflow.

One of the most important things to remember is to use quality control measures at every stage of the process. This includes using only sterile and clean materials, as well as ensuring that all instruments are properly calibrated. Additionally, it is important to follow established protocols carefully and avoid shortcuts that could lead to errors.

Concurrently, streamlining the process as much as you can is crucial. This includes using automation wherever possible and eliminating unnecessary steps. Additionally, it is important to make sure you're set up for success in order to keep the workflow running smoothly - so before you begin, take inventory of all supplies, equipment, and instruments and make sure you have enough of everything to get the job done without pausing.

It's also a good idea to review your goals and objectives for each of the 5 PCR workflow steps before you begin. The 5 steps are as follows:

  • Collection: The collection of biological samples from a patient or specimen. Methods vary based on the type of sample (saliva, blood, sputum, etc.)
  • Extraction: The isolation and separation of nucleic acids (DNA and/or RNA) from the collected sample, which will be used for PCR testing.
  • Amplification: "Cloning" specific segments of a nucleic acid sequence to create many more copies, thus improving testing accuracy.
  • Detection: The isolation and identification of pathogens or given conditions within the sample.
  • Storage: Having the proper equipment and processes in place to store samples over the long term for further research, or to store for transportation.

scientist working in PCR lab


Enjoy a free interactive asset to assist with your PCR workflow optimization.

Thomas Scientific provides a broad portfolio of solutions for each step of the PCR workflow. In addition offering products from leading scientific manufacturers, we also utilize a consultative and collaborative approach to making your workflow more efficient.

Click the link below to download a free interactive PCR workflow flyer and learn more about the products and services our team provides. You can also use the flyer to request a free workflow consultation from one of our Clinical Diagnostics or Bio Solutions experts!


Download the free interactive flyer

Latest Articles



In this insightful article, we'll delve into the intricacies of the EU GMP Annex 1 guidelines and explore how Ansell provides cutting-edge, compliant solutions to ensure your cleanroom meets and exceeds these stringent regulations.

Read More >


Protecting people, products, and processes during vaccine manufacturing

Most vaccine manufacturing processes depend on quality control at every step. Failures can be costly, dangerous, and compromise compliance. Cleanroom garments are necessary to protect both processes and products from contamination, while also protecting operators from the hazardous substances involved in manufacturing.

Read More >


Polycellu-LOSE: Why Cellulose is a Bad Fit for Biotechnology & Medical Device Critical Cleanrooms

Polyester Cellulose wipers have their place in controlled, but not critical cleanroom environments. Biotechnology and Medical Device cleanrooms demand cleaner contamination control solutions. Learn why you should LOSE your polycellulose wipes.

Read More >

Write A Comment