Protein Expression AC Separopore

Separopore® 4B-CL is crosslinked high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Separopore® 4B-CL is a proven base matrix for coupling affinity ligands. Separopore® 4B-CL provides good...

Cibacron Blue 3GA-Separopore® 6B-CL is an agarose conjugate in saline suspension used to searate and purify a variety of proteins. The ligand Cibacron Blue F3G-A is covalently coupled to Separopore® via chlorotriazine ring. The structure of the blue dye makes it a very versatile tool for separating...

Applications: Reduced glutathione is covalently linked Separopore® 4B for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing...

a-Lactose-Separopore® 4B-CL is used in protein chromatography, affinity chromatography and carbohydrate matrices. a-Lactose-Separopore® 4B-CL has been used to study microneme proteins and host-parasite cell surface receptors. Lactose-Separopore® 4B-CL may be used to isolate lactose binding proteins,...

Applications: Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilized streptavidin binds biotin and biotinylated substances and can be used for affinity...

Rapid immobilization of antibodies and other proteins to the matrix Immobilization of ligands through primary amines to purify recombinant proteins Immobilization of Protein A or Protein G for monoclonal antibody purification Pre-activated medium for immobilization of ligands containing a primary...

Applications: 2-Iminobiotin-Separopore® 6B-CL is used for the purification of avidin/streptavidin conjugates. Proteins coupled to (strept)avidin are more readily recovered in native form from 2-Iminobiotin-Separopore® than the biotin resin. It is also used for removal of free avidin from...

Wheat germ (Triticum vulgaris) agglutinin (WGA) is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine. WGA can bind oligosaccharides...

Cyanogen bromide-activated Separopore® 4B matrix is used for preparation of resins for affinity chromatography. Pre-activated medium is suitable for immobilization of ligands containing primary amines. Cyanogen bromide (CNBr) in base reacts with hydroxyl groups on Separopore® 4B to form cyanate...