| Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. |
| The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. |
| The immobilized streptavidin binds biotin and biotinylated substances and can be used for affinity chromatography applications. |
| The interaction between streptavidin and biotin is very strong and requires denaturating conditions for elution, which may destroy both the ligandand the sample. |
| Strptavidin-Separopore® 4B is extremely useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin. |
| It can be used in the purification of antigens, where biotinylated antibodies are incubated with antigen. The biotinylated antibody-antigen complex binds to Streptavidin Separopore from which the antigen can be eluted. |