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Buffer Solutions

A buffer is a solution created to resist pH changes when introducing internal and external influences. Buffers also maintain intercellular and extracellular pH within the physiological range, providing minimal interference in biological processes. At Thomas Scientific, we have an extensive range of buffers available, including sodium phosphate buffers, blue buffers and LDS sample buffers. We offer a selection of buffers and native gels that carry both positive and negative charges. Buffers are suitable for biochemical research applications such as cell cultivation, isolation of cells, and enzyme assays.

Buffer Solutions

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  • Orion® pH Buffers

    Thermo Scientific Orion

    Orion pH buffers are manufactured under ISO 9000 quality standards. Buffers are color-coded for ease of use, packaged with buffer/temperature tables for accurate calibrations and NIST traceability. All-in-One Buffer kit (1233M21) includes pint bottles of 4 (pink), 7 (yellow) and 10 (blue) buffers,...

  • Color-Coded pH Buffer Solutions

    chemForce® (powered by Thomas Scientific)

    Harmonized #: 3822.00.0002|5090 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Physical State: Liquid Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General Traceable to NIST standards

  • DPBS without Ca & Mg, 1X

    Quality Biological

    DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Applications DPBS can be used to provide a buffer system to maintain cell...

  • Color-Coded pH Buffers

    Ricca Chemical

    Standardized and certified traceable to NIST Standard Color-coded for quick reference Accuracy ±0.01 pH at 25°C pH temperature chart, lot numbers and expiration dates on each label pH 4.0 buffer is colored red, 7.0 buffer is yellow or green and 10.0 buffer is blue. Thomas numbers 4120R54,...

  • pH Buffer Solutions

    Oakton

    Choose from economical solution bottles or individually sealed pH buffer pouches. NIST traceable materials Labeled with pH vs. temperature tables for accurate calibration All Oakton pH buffer solutions are freshness dated, and standardized against NIST-traceable references to ensure quality....

  • Buffer Standard, pH 8.00

    Exaxol

    Meets requirements of both ISO 17025 and ISO 17034 Certified Reference Materials, made under the strictest standards for stability, homogeneity, and accuracy Tested to tolerances of 0.01 pH units Supported by detailed Certificate of Analysis including temperature variation values and stated...

  • Buffer, Reference Standard, pH 7.40 ± 0.01 at 25°C

    Ricca Chemical

    pH made Easy. Everything you need and want in a pH Buffer. NIST Traceability. Consistent, reliable performance. The tightest specification (+/- 0.01 pH unit) on all buffers. Long Expiration dates. Temperature conversion charts for all Buffers up to pH 11. Multiple sizes to fit your usage and budget...

  • 1X PBS, pH 7.4

    Quality Biological

    PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications PBS is...

  • Buffer Standard, pH 7.00

    Exaxol

    Meets requirements of both ISO 17025 and ISO 17034 Certified Reference Materials, made under the strictest standards for stability, homogeneity, and accuracy Tested to tolerances of 0.01 pH units Supported by detailed Certificate of Analysis including temperature variation values and stated...

  • Buffer Standard, pH 10.00

    Exaxol

    Meets requirements of both ISO 17025 and ISO 17034 Certified Reference Materials, made under the strictest standards for stability, homogeneity, and accuracy Tested to tolerances of 0.01 pH units Supported by detailed Certificate of Analysis including temperature variation values and stated...

  • Buffer Standard, pH 4.01

    Exaxol

    Meets requirements of both ISO 17025 and ISO 17034 Certified Reference Materials, made under the strictest standards for stability, homogeneity, and accuracy Tested to tolerances of 0.01 pH units Supported by detailed Certificate of Analysis including temperature variation values and stated...

  • Buffer Standard, pH 5.00

    Exaxol

    Meets requirements of both ISO 17025 and ISO 17034 Certified Reference Materials, made under the strictest standards for stability, homogeneity, and accuracy Tested to tolerances of 0.01 pH units Supported by detailed Certificate of Analysis including temperature variation values and stated...

  • pH Electrode Cleaning Kits

    Thermo Scientific Orion

    Extends the life of electrodes Prevents cross-contamination Removes bacterial or protein contaminants All kits contain four 1 oz. bottles of cleaning solution, a 15 mL beaker and a pipette. Sample Kit - includes one bottle each of cleaning solutions A, B, C, D Solution A - for removing protein...

  • Buffer Standard, pH 9.00

    Exaxol

    Meets requirements of both ISO 17025 and ISO 17034 Certified Reference Materials, made under the strictest standards for stability, homogeneity, and accuracy Tested to tolerances of 0.01 pH units Supported by detailed Certificate of Analysis including temperature variation values and stated...

  • 10X Phosphate Buffered Saline Solution

    IBI Scientific

    IBI’s Ultra-Pure Grade 10X PBS solution is ideal for use in sample preparations and as a wash buffer in general immunoassay applications. 10X PBS also performs very well in tissue and cell culture procedures. IBI’s 10X PBS solution is a modified Dulbecco buffer that does not contain...

  • Buffer, Reference Standard, pH 11.00 ± 0.01 at 25°C

    Ricca Chemical

    pH made Easy. Everything you need and want in a pH Buffer. NIST Traceability. Consistent, reliable performance. The tightest specification (+/- 0.01 pH unit) on all buffers. Long Expiration dates. Temperature conversion charts for all Buffers up to pH 11. Multiple sizes to fit your usage and budget...

  • pH Electrode Solutions

    Thermo Scientific Orion

    Electrode Storage Solution 475 mL plastic bottle pH of solution is 6.2 to 7.4 For short term storage of all electrodes for one day to a week. Electrode Filling Solution Internal filling solution for Ross-type electrodes (3M KCl). In packages of five 60 mL bottles.

  • 10% Saline Solution, Sterile

    Teknova

    10% Saline Solution, Sterile. 10 mL Per Tube, 25 Per Pack. 4 pack.

  • Buffer, Reference Standard, pH 13.00 ± 0.01 at 25°C

    Ricca Chemical

    pH made Easy. Everything you need and want in a pH Buffer. NIST Traceability. Consistent, reliable performance. The tightest specification (+/- 0.01 pH unit) on all buffers. Long Expiration dates. Multiple sizes to fit your usage and budget constraints. All combined in one of the most comprehensive...

  • Traceable® pH Buffer Standards (CRM)

    Thomas®

    The most accurate pH Standards available anywhere Certified Reference Material Traceable® Certificate supplied Highest accuracy available Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffers at...

  • Fluka Sodium hydroxide solution volumetric, 8 M NaOH (8N)

    Honeywell Research Chemicals

    Chemical name: Sodium hydroxide Product Brand: Analytical Density (g/cm3): 1.275 Molecular weight: 40 Molecular formula: HNaO UN Number: UN 1824

  • Traceable® ph Buffer Standards (CRM), Color Coded

    labForce® (powered by Thomas Scientific)

    The MOST accurate pH Standards available anywhere now color-coded for convenience Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffer at 25°C is ±0.010 pH—the most precise available....

  • TE Buffer [1X]

    G-Biosciences

    TE Buffer [1X], pH 8.0, Low EDTA (Tris-EDTA; 10mM Tris base, 0.1mM EDTA) are available in different sizes. Other concentrations of TE Buffer are offered, including a 100X concentration and a 10X concentration. 10X and 100X regular TE Buffers are also available Features Low EDTA [0.1mM] DNase and...

  • Clear pH Buffer Solutions

    chemForce® (powered by Thomas Scientific)

    Harmonized #: 2806.10.0000|0000 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Appearance: Colorless Physical State: Liquid Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General

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What is nuclease free water?

Nuclease-free water is highly purified water removed of nucleases. This type of water is essential in molecular biology experiments, such as PCR, RNA sequencing, or DNA extraction, where the integrity of nucleic acids is crucial. By using nuclease-free water, scientists ensure that their samples won't be degraded by unwanted enzymes, preserving the accuracy and reliability of their experiments.

Why is nuclease free water used in PCR?

Nuclease-free water is essential in PCR (Polymerase Chain Reaction) to prevent degradation of the DNA template and primers. Nucleases are enzymes that can degrade DNA or RNA, which would compromise the accuracy and success of the PCR process.
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