Buffer Solutions

A buffer is a solution created to resist pH changes when introducing internal and external influences. Buffers also maintain intercellular and extracellular pH within the physiological range, providing minimal interference in biological processes. At Thomas Scientific, we have an extensive range of buffers available, including sodium phosphate buffers, blue buffers and LDS sample buffers. We offer a selection of buffers and native gels that carry both positive and negative charges. Buffers are suitable for biochemical research applications such as cell cultivation, isolation of cells, and enzyme assays.

Buffer Solutions

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  • TAE (Tris-Acetate EDTA), 50X, pH 8.3

    Sterile liquid electrophoresis buffer concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. TAE buffer is used in agarose gel electrophoresis and Polyacrylamide gels both as a buffer and a component of a gel. Can be used for both genomic...

  • Orion® pH Buffers

    Thermo Scientific Orion

    Orion pH buffers are manufactured under ISO 9000 quality standards. Buffers are color-coded for ease of use, packaged with buffer/temperature tables for accurate calibrations and NIST traceability. All-in-One Buffer kit (1233M21) includes pint bottles of 4 (pink), 7 (yellow) and 10 (blue) buffers,...

  • TAE Buffer, Solution

    Bio Basic Inc.

    TAE buffer 25x solution: TAE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but dsDNA runs faster with TAE buffer. This 25x and 50x concentrate can be easily diluted with molecular biology grade water to 1x...

  • TBST [10X]; Tris buffered saline with Tween-20

     A 10X Concentrated solution of Tris Buffered Saline with Tween® 20.    The 10X Concentration is 100mM Tris.HCl, 150mM NaCl, 0.5% Tween® 20 at pH7.5.   The 1X Concentration is 10mM Tris.HCl, 15mM NaCl, 0.05% Tween® 20 at pH7.5.   TBST is commonly used...

  • Color-Coded pH Buffers

    Standardized and certified traceable to NIST Standard Color-coded for quick reference Accuracy ±0.01 pH at 25°C pH temperature chart, lot numbers and expiration dates on each label pH 4.0 buffer is colored red, 7.0 buffer is yellow or green and 10.0 buffer is blue. Thomas numbers 4120R54,...

  • pH Buffer Solutions

    Choose from economical solution bottles or individually sealed pH buffer pouches. NIST traceable materials Labeled with pH vs. temperature tables for accurate calibration All Oakton pH buffer solutions are freshness dated, and standardized against NIST-traceable references to ensure quality....

  • ACK Lysing Buffer

    Quality Biological

    Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow. Applications ACK Lysing Buffer is used to lyse red blood cells in samples, such as EDTA-treated whole...

  • Color-Coded pH Buffer Solutions

    chemForce® (powered by Thomas Scientific)

    Harmonized #: 3822.00.0002|5090 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Physical State: Liquid Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General Traceable to NIST standards

  • Buffer, Reference Standard, pH 1.68 ± 0.01 at 25°C

    NIST traceability Wide choice of sizes Long life Reference Standard pH Buffers make pH easy. We offer consistent, reliable performance and NIST traceability. All buffers offer tight specifications (±0.01 ph unit) and a long shelf life. Temperature conversion charts are available for all...

  • Buffer, Water Hardness, without Magnesium EDTA

    Formula: Mixture

  • Buffer Standard, pH 10.00

    Meets requirements of both ISO 17025 and ISO 17034 Certified Reference Materials, made under the strictest standards for stability, homogeneity, and accuracy Tested to tolerances of 0.01 pH units Supported by detailed Certificate of Analysis including temperature variation values and stated...

  • Technical pH Buffers

    Mettler Toledo

    All METTLER TOLEDO calibration and cleaning solutions are available in handy bottles with no-fuss labels. Specialties for specific requirements Using our sachets guarantees fresh solution for every calibration as well as maximum ease of use. Every sachet box comes with a printed certificate. Rely on...

  • Buffer, Reference Standard, pH 7.00 ± 0.01 at 25°C

    pH made Easy. Everything you need and want in a pH Buffer. NIST Traceability. Consistent, reliable performance. The tightest specification (+/- 0.01 pH unit) on all buffers. Long Expiration dates. Temperature conversion charts for all Buffers up to pH 11. Multiple sizes to fit your usage and budget...

  • Glycine

    Bio Basic Inc.

    Glycine: Molecular Formula: C2H5NO2 Molecular Weight: 75.07 CAS Number: 56-40-6 pI: 6.06 pKa: for the α carboxyl group: 2.35; and for the α amino group: 9.78 Melting Point: 233-290 oC Glycine does not have a chiral center. Therefore, it does not have a D- or L- isomer designation. This...

  • 1M TRIS-HCl, pH 7.5

    Quality Biological

    Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its buffering range is between 7.1-8.9. The lower pH 7.4 can be associated more with maintaining RNA, however, the pH 8.0 works equally as well with both DNA and RNA nucleic acids. Applications Tris...

  • Traceable® pH Buffer Standards (CRM)

    The most accurate pH Standards available anywhere Certified Reference Material Traceable® Certificate supplied Highest accuracy available Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffers at...

  • Traceable® ph Buffer Standards (CRM), Color Coded

    labForce® (powered by Thomas Scientific)

    The MOST accurate pH Standards available anywhere now color-coded for convenience Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffer at 25°C is ±0.010 pH—the most precise available....

  • 10X Tris-Borate-EDTA (TBE) (0.9 M Tris-Borate 0.01 M EDTA pH 8.3)

    This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Tris-Borate EDTA (TBE) consists of 0.9 M Tris-Borate, 0.01 M EDTA at a pH of 8.3. Meticulously prepared using ultra pure reagents...

  • Buffer, pH 7.00, Yellow, Standardized

    Composition: Water 98.73%, Sodium Phosphate Dibasic 0.79%, Potassium Dihydrogen Phosphate 0.47%, Sodium Azide 0.01%, Brilliant Yellow 0% Boiling Point: Approximately 100°C Density: 1 Melting Point: Approximately 0°C Color: Yellow liquid Physical State: Liquid pH Range: 7 Solubility Information:...

  • TAE [50X] (Tris-Acetate-EDTA)

    Features 2M Tris-Acetate, 0.05M EDTA (pH8.3)    

  • Clear pH Buffer Solutions

    chemForce® (powered by Thomas Scientific)

    Harmonized #: 2806.10.0000|0000 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Appearance: Colorless Physical State: Liquid Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General

  • Color-Coded pH Buffer Solution Set

    chemForce® (powered by Thomas Scientific)

    Size: 3 x 500 mL Harmonized #: 3822.00.0002|5090 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Appearance: Color coded Physical State: Liquid pH: 4, 7, 10 Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General

  • Traceable® ColorCode pH One-Shot™ Assorted

    labForce® (powered by Thomas Scientific)

    The MOST accurate pH Standards available anywhere now color-coded for convenience Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffer at 25°C is ±0.010 pH—the most precise available....

  • High-Accuracy pH Buffer Solutions

    pH Calibration buffers for tight tolerances For meters with 0.001 resolution Buffers are color-coded for easy visual identification.

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What is nuclease free water?

Nuclease-free water is highly purified water removed of nucleases. This type of water is essential in molecular biology experiments, such as PCR, RNA sequencing, or DNA extraction, where the integrity of nucleic acids is crucial. By using nuclease-free water, scientists ensure that their samples won't be degraded by unwanted enzymes, preserving the accuracy and reliability of their experiments.

Why is nuclease free water used in PCR?

Nuclease-free water is essential in PCR (Polymerase Chain Reaction) to prevent degradation of the DNA template and primers. Nucleases are enzymes that can degrade DNA or RNA, which would compromise the accuracy and success of the PCR process.