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Buffer Solutions

A buffer is a solution created to resist pH changes when introducing internal and external influences. Buffers also maintain intercellular and extracellular pH within the physiological range, providing minimal interference in biological processes. At Thomas Scientific, we have an extensive range of buffers available, including sodium phosphate buffers, blue buffers and LDS sample buffers. We offer a selection of buffers and native gels that carry both positive and negative charges. Buffers are suitable for biochemical research applications such as cell cultivation, isolation of cells, and enzyme assays.

Buffer Solutions

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  • Tris Base

    Vion Biosciences

    Bioreagent grade tris base for molecular biology, Dnase-free, Rnase-free, = 99.5%

  • Color-Coded pH Buffer Solutions

    chemForce® (powered by Thomas Scientific)

    Harmonized #: 3822.00.0002|5090 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Physical State: Liquid Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General Traceable to NIST standards

  • Tris Base, Molecular Biology Grade

    MilliporeSigma

    Widely used as a biological buffer (absorbance: Synonyms: Tris(hydroxymethyl)aminomethane; tris(Hydroxymethyl)aminomethane; 2-Amino-2-(hydroxymethyl)-1,3-propanediol; THAM; Tris base; Trometamol Molecular Formula: C4H11NO3 Molecular Weight: 121.14 Linear Structural Formula: NH2C(CH2OH)3 MDL Number:...

  • Color-Coded pH Buffers

    Ricca Chemical

    Standardized and certified traceable to NIST Standard Color-coded for quick reference Accuracy ±0.01 pH at 25°C pH temperature chart, lot numbers and expiration dates on each label pH 4.0 buffer is colored red, 7.0 buffer is yellow or green and 10.0 buffer is blue. Thomas numbers 4120R54,...

  • Orion® pH Buffers

    Thermo Scientific Orion

    Orion pH buffers are manufactured under ISO 9000 quality standards. Buffers are color-coded for ease of use, packaged with buffer/temperature tables for accurate calibrations and NIST traceability. All-in-One Buffer kit (1233M21) includes pint bottles of 4 (pink), 7 (yellow) and 10 (blue) buffers,...

  • VeriSpec® pH Buffer and Conductivity Standard

    Ricca Chemical

    Ricca’s VeriSpec® pH Buffer and Conductivity Standard Certified Reference Materials are ISO 17025/17034 accredited to provide the highest level of quality assurance. These products have undergone rigorous evaluations of manufacturing methods, quality control, stability, and homogeneity. As...

  • 1X PBS, pH 7.4

    Quality Biological

    PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications PBS is...

  • 5M NaCl (Sodium Chloride)

    Growcells

    Suitable for the removal of small nucleic acids fragments from plasmid DNA preparations and precipitaiton of DNA from SDS-containing samples. Widely used in protein crystallization and in the determination of heavy and transition metals in biochemical samples by ion chromatography.

  • Fluka Calcium chloride solution volumetric, 1.0 M CaCl2

    Honeywell Research Chemicals

    Chemical name: Calcium Chloride Product Brand: Analytical Material form: Solution Density (g/cm3): 1.09 Molecular weight: 110.98 Molecular formula: CaCl2 Chemical purity: =98% UN Number:

  • Buffer Solution, pH 4.00 (Color Coded Red)

    GFS Chemicals

    pH (@ 25°C) 4.00 +/- 0.02 NIST Traceable

  • TE Buffer, pH 8.0

    Bio Basic Inc.

    TE buffer pH 8.0 sterile by filtration

  • Buffer Solution, pH 7.00 (Color Coded Yellow)

    GFS Chemicals

    pH (@ 25°C) 7.00 +/- 0.02 NIST Traceable

  • Buffer Solution, pH 10.00 (Color Coded Blue)

    GFS Chemicals

    pH (@ 25°C) 10.00 +/- 0.03 NIST Traceable

  • pH Buffer Solutions

    AquaPhoenix Scientific

    pH Buffers are manufactured under ISO 9001 quality standards and are NIST-traceable. Induction sealed caps ensure product integrity and decrease the possibility of leaks. Buffers should be used regularly to calibrate your pH meters.

  • Tris(hydroxymethyl)aminomethane, ACS, 99.8-100.1%

    BeanTown Chemical

    CAS: 77-86-1 EC No: 201-064-4 MDL No: MFCD00004679 RTECS: TY2900000 Crystalline Molecular Formula: C4H11NO3 MW: 121.14 Melting Point: 169° Boiling Point: 288° (decomposes below boiling point) Density (g/mL): 1.353

  • 40% (v/v) Glycerol Solution, Sterile

    Teknova

    40% (v/v) Glycerol

  • Proteinase K with Storage Buffer Set

    Zymo Research Corporation

    Proteinase K is a stable serine protease with broad substrate specificity and will degrade many proteins in their native conformation even in the presence of detergents (e.g., SDS). The enzyme is frequently used in molecular biology applications to digest unwanted proteins such as nucleases from DNA...

  • Fluka Ethylenediaminetetraacetic acid dipotassium magnesium salt for complexometry, =98%

    Honeywell Research Chemicals

    Chemical name: Ethylenediaminetetraacetic acid dipotassium magnesium salt Chemical synonyms: EDTA-K2Mg Product Brand: Analytical Molecular weight: 426.74 Molecular formula: C10H12K2MgN2O8 · 2H2O Chemical purity: =98% UN Number:

  • 1M TRIS-HCl, pH 7.5

    Quality Biological

    Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its buffering range is between 7.1-8.9. The lower pH 7.4 can be associated more with maintaining RNA, however, the pH 8.0 works equally as well with both DNA and RNA nucleic acids. Applications Tris...

  • Buffer, Reference Standard, pH 3.00 ± 0.01 at 25°C

    Ricca Chemical

    pH made Easy. Everything you need and want in a pH Buffer. NIST Traceability. Consistent, reliable performance. The tightest specification (+/- 0.01 pH unit) on all buffers. Long Expiration dates. Temperature conversion charts for all Buffers up to pH 11. Multiple sizes to fit your usage and budget...

  • Traceable® pH Buffer Standards (CRM)

    Thomas®

    The most accurate pH Standards available anywhere Certified Reference Material Traceable® Certificate supplied Highest accuracy available Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffers at...

  • Buffer Standard, pH 7.00

    Exaxol

    Meets requirements of both ISO 17025 and ISO 17034 Certified Reference Materials, made under the strictest standards for stability, homogeneity, and accuracy Tested to tolerances of 0.01 pH units Supported by detailed Certificate of Analysis including temperature variation values and stated...

  • Traceable® ph Buffer Standards (CRM), Color Coded

    labForce® (powered by Thomas Scientific)

    The MOST accurate pH Standards available anywhere now color-coded for convenience Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffer at 25°C is ±0.010 pH—the most precise available....

  • Clear pH Buffer Solutions

    chemForce® (powered by Thomas Scientific)

    Harmonized #: 2806.10.0000|0000 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Appearance: Colorless Physical State: Liquid Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General

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What is nuclease free water?

Nuclease-free water is highly purified water removed of nucleases. This type of water is essential in molecular biology experiments, such as PCR, RNA sequencing, or DNA extraction, where the integrity of nucleic acids is crucial. By using nuclease-free water, scientists ensure that their samples won't be degraded by unwanted enzymes, preserving the accuracy and reliability of their experiments.

Why is nuclease free water used in PCR?

Nuclease-free water is essential in PCR (Polymerase Chain Reaction) to prevent degradation of the DNA template and primers. Nucleases are enzymes that can degrade DNA or RNA, which would compromise the accuracy and success of the PCR process.
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