Buffer Solutions

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A buffer is a solution created to resist pH changes when introducing internal and external influences. Buffers also maintain intercellular and extracellular pH within the physiological range, providing minimal interference in biological processes. At Thomas Scientific, we have an extensive range of buffers available, including sodium phosphate buffers, blue buffers and LDS sample buffers. We offer a selection of buffers and native gels that carry both positive and negative charges. Buffers are suitable for biochemical research applications such as cell cultivation, isolation of cells, and enzyme assays.

Buffer Solutions

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  • Buffer, Phosphate, pH 7.2

    For Biochemical Oxygen Demand (BOD) testing Formula: Mixture

  • Color-Coded pH Buffers

    Standardized and certified traceable to NIST Standard Color-coded for quick reference Accuracy ±0.01 pH at 25°C pH temperature chart, lot numbers and expiration dates on each label pH 4.0 buffer is colored red, 7.0 buffer is yellow or green and 10.0 buffer is blue. Thomas numbers 4120R54,...

  • Color-Coded pH Buffer Solutions

    chemForce® (powered by Thomas Scientific)

    Harmonized #: 3822.00.0002|5090 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Physical State: Liquid Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General Traceable to NIST standards

  • VeriSpec® pH Buffer and Conductivity Standard

    Ricca’s VeriSpec® pH Buffer and Conductivity Standard Certified Reference Materials are ISO 17025/17034 accredited to provide the highest level of quality assurance. These products have undergone rigorous evaluations of manufacturing methods, quality control, stability, and homogeneity. As...

  • Glycine

    Bio Basic Inc.

    Glycine: Molecular Formula: C2H5NO2 Molecular Weight: 75.07 CAS Number: 56-40-6 pI: 6.06 pKa: for the α carboxyl group: 2.35; and for the α amino group: 9.78 Melting Point: 233-290 oC Glycine does not have a chiral center. Therefore, it does not have a D- or L- isomer designation. This...

  • pH Buffer Solutions

    Choose from economical solution bottles or individually sealed pH buffer pouches. NIST traceable materials Labeled with pH vs. temperature tables for accurate calibration All Oakton pH buffer solutions are freshness dated, and standardized against NIST-traceable references to ensure quality....

  • SIGMA Phosphate buffered saline, 10x concentrate, BioReagent, suitable for cell culture, for molecular biology

    Synonyms: PBS Other Notes: When diluted to a 1X concentration, this product will yield a phosphate buffered saline solution with a phosphate buffer concentration of 0.01M and a sodium chloride concentration of 0.154M. The solution pH will be 7.4. Packaging: 4L package size comes in a box with a...

  • 1X PBS Solution, pH 7.4, Sterile

    This is a GMP-grade product that is manufactured in compliance with our Quality Management System in ISO 13485 certified facilities., 135mM Sodium Chloride, 2.7mM Potassium Chloride, 4.3mM Disodium Phosphate, 1.4mM Monopotassium Phosphate

  • 1X PBS, pH 7.2

    Quality Biological

    PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications PBS is...

  • pH Buffers

    Various pH buffers for pH probe calibration. Buffer cases are available with a single buffer type or with an assortment of buffers (2 pints of pH 4, 7, and 10 buffers).

  • 1X TE pH 8.0 (10 mM Tris HCl, 1.0 mM EDTA) DEPC TREATED, 1L, Liquid (sterile filtered)

    This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 10 mM Tris HCl and 1.0 mM EDTA at a pH of 8.0. Meticulously prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionized water.

  • 1M TRIS-HCl, pH 7.5

    Quality Biological

    Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its buffering range is between 7.1-8.9. The lower pH 7.4 can be associated more with maintaining RNA, however, the pH 8.0 works equally as well with both DNA and RNA nucleic acids. Applications Tris...

  • 0.5M EDTA, pH 8.0

    Quality Biological

    EDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and reagents,...

  • 1X PBS, pH 7.4

    Quality Biological

    PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications PBS is...

  • Traceable® pH Buffer Standards (CRM)

    The most accurate pH Standards available anywhere Certified Reference Material Traceable® Certificate supplied Highest accuracy available Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffers at...

  • Orion® pH Buffer & Electrode Rinse Solution Pouches

    Accurate calibration Sealed buffer pouch provides disposable convenience More accurate first time measurements Easier to use than conventional liquid buffers; no secondary reservoir required. All buffers are certified NIST traceable; used pouch can be attached to permanent batch records. Each buffer...

  • 1X PBS (Phosphate Buffered Saline)

    Ready to use, sterile 1X Phosphate-Buffered Saline solution prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Balanced salt solution used in many biomolecular applications. Ultra pure quality makes buffer ideal for use in a variety of cell culture...

  • TAE [50X] (Tris-Acetate-EDTA)

    Features 2M Tris-Acetate, 0.05M EDTA (pH8.3)    

  • Buffer Solutions

    Avantor - J.T.Baker®

    Ready-to-use buffers Accurate within ±0.01 pH unit of specified value, at 25°C In 500 mL polyethylene squeeze bottles

  • High-Accuracy pH Buffer Solutions

    pH Calibration buffers for tight tolerances For meters with 0.001 resolution Buffers are color-coded for easy visual identification.

  • Certipur® Buffer Solution, pH 7.00

    pH: 7.0 (H2O, 20°C) Solubility: (20°C) soluble Melting Point: -5°C Boiling Point: 109°C Density: 1.01 g/cm3 (20°C)

  • Buffer solution pH 10.00

    109409 Buffer solution pH 10.00 (boric acid/potassium chloride/sodium hydroxide), traceable to SRM from NIST and PTB pH 10.00 (25°C) Certipur®

  • NPP (1X) ELISA ALKALINE PHOSPHATASE SUBSTRATE, 100mL, Liquid - clear to pale yellow

    NPP (1X) ELISA ALKALINE PHOSPHATASE SUBSTRATE, 100mL, Liquid - clear to pale yellow, Expiration date is one (1) year from date of opening.

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What is nuclease free water?

Nuclease-free water is highly purified water removed of nucleases. This type of water is essential in molecular biology experiments, such as PCR, RNA sequencing, or DNA extraction, where the integrity of nucleic acids is crucial. By using nuclease-free water, scientists ensure that their samples won't be degraded by unwanted enzymes, preserving the accuracy and reliability of their experiments.

Why is nuclease free water used in PCR?

Nuclease-free water is essential in PCR (Polymerase Chain Reaction) to prevent degradation of the DNA template and primers. Nucleases are enzymes that can degrade DNA or RNA, which would compromise the accuracy and success of the PCR process.