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bioWORLD

NHS-activated Separopore® (Agarose) 4B-CL

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Rapid immobilization of antibodies and other proteins to the matrix
Immobilization of ligands through primary amines to purify recombinant proteins
Immobilization of Protein A or Protein G for monoclonal antibody purification
Pre-activated medium for immobilization of ligands containing a primary amino group
N-hydroxyl succinimide (NHS) ester forms a stable amide (peptide) bond with the primary amines of ligands
The stability of an amide bond at high pH (up to pH 13) allows for a very stringent wash protocol during affinity purification using NHS-activated ligand coupling
Coupling can be carried out in neutral pH which makes it ideal for immobilization of antibodies, antigens, and other proteins sensitive to pH extremes
Coupling reaction can also be carried out in organic solvents
Provides a 10 atom spacer arm upon ligand coupling
Coupling completed after 2 – 4 h at room temperature
NHS-Activated Separopore® provides a valuable tool for affinity purification of antibodies, antigens and other biomolecules

Group to be coupled:	Primary -NH₂
Matrix:	Separopore® 4B-CL (highly crosslinked agarose beads, 4%)
Particle size range:	52 – 165 µm
Molecular weight range:	6 x 10⁴ – 2 x 10⁷
Chemical linkage of ligand:	Amide bond
Spacer arm:	6-aminocaproic acid, 10 atoms
Substitution:	>18 µmol NHS / ml drained gel
pH stability:	3 – 13 (ligand dependent)
Coupling conditions:	pH 6 – 9; Temp: 4 – 25 °C
Storage:	2 – 8 °C
Flow rate specifications:	70 – 140 cm / h
Supplied as a pre-swelled gel suspension in 100% isopropanol to protect the active groups

list price/quantity	total
$395.82 /EA (1/EA)	$0.00

list price/quantity	total
$591.75 /EA (1/EA)	$0.00

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