Tissue Culture Media

This 1:1 mixture of Dulbecco’s Modified Eagle Medium and Ham’s F-12 media is ideal for culture of a variety of cell types under low serum conditions. Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the...

HEPES 1M Solution. Used in cell culture media to maintain the pH levels of basal media.

Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of...

20X Saline-Sodium-Phosphate-EDTA (SSPE) buffer has applications in nucleic acid hybridization and other molecular biology work.

A less complex and extensively modified version of Medium 199, CMRL-1066 was designed initially for use with L-strain cells in unsupplemented culture. It has been used to maintain monolayer growth of permanent cell lines for years without protein supplementation. Although developed for use in...

EMEM (Eagle’s Minimal Essential Medium) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations Without L-Glutamine Applications EMEM has a higher amino acid concentration and 2 to 5 times greater vitamin...

Hanks' Balanced Salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

Ampicillin is used to select for mutated or transformed cells that are ampicillin resistant (Amp or Amp R ) [i.e., carry the ß-lactamase ( bla ) gene]. Typically, low-copy-number plasmids require less ampicillin than high-copy-number plasmids. In some situations (e.g., if extracellular lactamase...

Lymphocyte Separation Medium (LSM) was originally designed for the in vitro isolation of lymphocytes from diluted whole blood.  It is a sterile, iso-osmotic polysucrose and diatrizoate solution with low viscosity and a density of 1.077-1.080 g/mL at 20ºC. Complete Certificate of Analysis...

Applications The FBS in the Cryopreservation Medium improves the post-freezing recovery and survival after cryopreservation, while DMSO preserves the cells during freeze. Cryopreservation Medium with Fetal Bovine Serum (FBS) and Calf Serum is a complete cryopreservation medium with 10% DMSO.

The 35° angled cassette features a large writing surface and two side panels ideal for labeling. The one-piece design allows for the lids to be opened and closed repeatedly without the lid getting detached from the base. The lid securely locks into the base and keeps samples safe and secure. The 35°...

Gentamicin is a broad-spectrum bactericidal antibiotic that inhibits the growth of Gram-negative bacteria, Gram-positive bacteria, and mycoplasma. It inhibits protein synthesis by binding to the L6 protein of the 50S ribosomal subunit. It is used as a selection agent in molecular biology and cell...

Sodium bicarbonate Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7.2 - 7.4 in a culture environment.  Bicarbonate is a weak buffer with that makes maintaining a pH range of 7.2 - 7.6 somewhat difficult, but it is non-toxic, and is a nutrient...

Collagen I, found in most tissues and organs, is most plentiful in dermis, tendon, and bone. It is an integral part of the framework that holds cells and tissues together and has been recognized as a useful matrix for improving cell culture. in vitro use of collagen can exert effects on the...

Preparation Method: Add 5% less distilled water than the desired total volume to a mixing container just large enough to hold the final media volume. Add powdered medium to room temperature (20 -30°C) water with gentle stirring. Do not heat water. Rinse inside of pouch to remove all traces of...

Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of...

Glucose is a main source of energy for living organisms and is used as a nutrient source in cell culture media.  This 45% (w/v) solution has been to ensure no cytotoxicity.

Cellvation Cryopreservation Media is animal component free and has been shown to preserve the potential of stem cell for differentiation. This medium is recommended for the preservation of stem cells, hepatocytes, tissue samples, and a wide variety of cell types and established cell lines....

with 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted...

Dulbecco’s Modified Eagle Medium (DMEM) w/ 4.5gms Glucose per litre, L- Alanyl-L-Glutamine, 3.7 gms per litre Sodium bicarbonate and Sodium pyruvate. Contains stabililzed dipeptide of L-Glutamine.

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport...

Corning transfectagro Reduced Serum Medium is designed to maximize the growth of a variety of cell types under reduced serum conditions, generally allowing for at least a 50% reduction in serum use with minimal adaptation. transfectagro has also been proven to improve transient transfection...

Corning glutagro™, phenol red Named for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy’s 5A containing an increased level of inositol. Examples of cell types for which this medium is appropriate include mammalian myeloma and...

Catalase Reagent Droppers are used in a qualitative procedure for determining catalase activity by bacteria. The enzyme catalase is preset in most aerobic and facultatively anaerobic bacteria, with the major exception of streptococci and enterococci. The enzyme catalase decomposes hydrogen peroxide...