Gentamicin is a broad-spectrum bactericidal antibiotic that inhibits the growth of Gram-negative bacteria, Gram-positive bacteria, and mycoplasma. It inhibits protein synthesis by binding to the L6 protein of the 50S ribosomal subunit.
It is used as a selection agent in molecular biology and cell culture for strains and plasmids containing a gentamicin-resistance gene (aacC1 confers resistance to gentamycin only, and aacA-aphD confers resistance to gentamicin and kanamycin in two separate domains).
10 µg/mL gentamicin is the most commonly used concentration.
Contains 30 mg/mL or 100 mg/mL gentamicin sulfate.