Tissue Culture Media

with 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted...

10x PBS Solution (Phosphate Buffered Saline): This PBS formulation is recommended for use in immunohistology protocols. Each package contains 4 L of 10 concentrated PBS or 40 liters of 1 x solution. Each 1 x PBS solution contains 137 mM Sodium Chloride, 2.7mM Potassium Chloride and 10mM Phosphate...

This 1:1 mixture of Dulbecco's Modified Eagle Medium and Ham's F-12 media is ideal for culture of a variety of cell types under low serum conditions. Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the...

Phosphate Buffered Saline, pH 7.2 1X. Commonly used for tissue disaggregation and monolayer dispersal.

Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of...

Corning BioCoat cell culture inserts are pre-coated with extracellular matrix proteins for applications requiring a protein-coated cell surface, such as cell adhesion, growth, invasion, migration and/or differentiation. Coatings include Corning Matrigel® matrix, Fibronectin, Collagen, and...

Trypsin is used to enzymatically release adherent cells from tissue culture plates for passaging.  Divalent cations such as calcium and magnesium, which are often present in the cell culture environment, inhibit this action.  EDTA sequesters these ions and thus enhances the efficacy of...

Water, Sterile, Molecular Biology Grade Endotoxin tested, DEPC treated, Nuclease and Protease free. Used for molecular biology applications like DNA/RNA purification, protein applications and experiments involving RNA.

BBL™CHROMagar™ Orientation medium is a nonselective differentiated medium for the isolation, differentiation and enumeration of urinary tract pathogens. BBL CHROMagar Orientation medium allows for the differentiation and identification of Escherichia coli and Enterococcus without...

Ampicillin is used to select for mutated or transformed cells that are ampicillin resistant (Amp or Amp R ) [i.e., carry the ß-lactamase ( bla ) gene]. Typically, low-copy-number plasmids require less ampicillin than high-copy-number plasmids. In some situations (e.g., if extracellular lactamase...

Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of...

McCoy's 5A medium was originally formulated to meet the culture requirements of Novikoff Hepatoma cells, and has been found to support the indefinite proliferation of Walker 256 carcinoma cells along with a wide range of human and rat normal and transformed cells. 

BioRich 1 with 10 common anchorage dependent cell lines commonly show increased yields from 8% to 114% when compared to standard media in normal serum-containing systems. BioRich 1 also shows elevated growth of hybridoma lines at reduced serum concentrations when compared to normal base media....

Growth Promotion Test Suspensions provide a quality control challenge to each batch or lot of medium, ensuring its growth promoting qualities, whether it is prepared internally from basic ingredients or purchased commercially prepared. Inoculate each batch or lot of medium, directly without...

Specialized for use in insect cell culture Poloxamer 188 is a surfactant used in cell culture to protect cells in suspension against possible damage during transfer, freezing and thawing, and stirring. It is a non-ionic detergent with an average molecular weight of 8400Da consisting of a central...

Dehydrated culture media Refrigerate, store at 2° to 8°C

This modification of Dulbecco's MEM was developed by Dr. Iscove and his coworkers to assist in their studies of hematopoietic stem cells. They modified Dulbecco's MEM by the addition of HEPES buffer, selenium, additional amino acids, vitamins and sodium pyruvate, and potassium nitrate...

Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. We offer a variety of DMEM modifications for a range of cell culture applications. Applications DMEM is one of the most widely used modifications of Basal Medium Eagle (BME). DMEM...

Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations. Applications MEM was developed by Dr. Harry Eagle to overcome problems encountered when attempting to grow normal...

Ham's F-10 is a classical media designed to support the growth of a variety of cell types, including HeLa, Chinese Hamster Ovary (CHO), primary cells from tissue explants and various types of human diploid cells. 

Laminin (LM), a major component of basement membranes, is a multifunctional glycoprotein that is used as a substrate to culture and maintain differentiated function of a wide variety of cells. Laminin has been shown in culture to stimulate neurite outgrowth, promote cell attachment, chemotaxis, cell...

Corning extracellular matrices (ECMs) enable researchers to mimic in vivo environments for 2D and 3D cell culture applications.

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L sodium bicarbonate With HEPES buffer Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence...