Description: Mature, recombinant Ebola Virus Soluble Glycoprotein (EBOV sGP) containing a c-terminal HIS tag, is supplied as purified protein. EBOV sGP is produced in mammalian cells and is purified by FPLC
Size: 100 µg of protein is supplied in PBS (supplemented with glycerol, arginine and glutamic acid) at a concentration of 1.943 mg/mL. The theoretical molecular weight of the protein is ~43 kDa. Because of the glycosylated nature of this protein, migration in an SDS-PAGE gel is slowed resulting in a broad, diffuse band representing differing glycosylation forms.
Relevance: Recombinant glycoprotein provides a means for antibody development, control protein for testing, and a tool to enhance research.
Western Blot: Quality control testing demonstrates strong detection of soluble GP under reduced conditions down to 50 ng when using IBT’s Rabbit anti-EBOV sGP polyclonal antibody (cat 365-001) at 0.1 ug/mL.
Storage: 2-3 weeks at -20°C, -80°C long term.
Intended for research use only, not for human, therapeutic, or diagnostic applications.