Macrophage colony-stimulating factor (M-CSF) is a hematopoietic growth factor with broad production across different cell types (1). The regulation of monocyte and macrophage development to maintain homeostasis is primarily governed by M-CSF, which is generated by stromal cells present in both the bloodstream and tissues. Mature mononuclear phagocytes, in response, possess CSF-1 receptors (CSF-1R) and eliminate circulating CSF-1. This process creates a feedback mechanism responsible for diminishing monocyte proliferation (2, 3, 4). M-CSF also exerts its effects by interacting with CSF1R, influencing various processes related to immunology, bone health, fertility, and pregnancy regulation (5). Human M-CSF has cross reactivity to mouse cells, whereas mouse M-CSF does not exhibit activity on human cells (6).
Bioactivity Assay:Proliferation of NFS-60 cell ; ED50 =10 ng/mL; Bioactivity =1.02 x 10^5 IU/µg