HPLC Columns & Fittings

HPLC columns provide high-resolution separation for analytical and preparative chromatography applications. HPLC gravity chromatography columns deliver consistent performance for complex sample analysis. HPLC gravity columns optimize flow rates and efficiency, ensuring reproducible pharmaceutical, environmental, and biochemical research results. For high-throughput applications, large HPLC columns support increased sample capacity and purification needs. Explore HPLC columns for precise and reliable chromatographic separations.

HPLC Columns & Fittings

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  • Ascentis® Express Phenyl-Hexyl

    The Phenyl-Hexyl phase has unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity. The Phenyl-Hexyl phase also tend to exhibit good shape selectivity,...

  • SeQuant® ZIC®-HILIC

    MilliporeSigma

    SeQuant ® ZIC ® -HILIC is your ideal choice for separation of polar and hydrophilic compounds. The zwitterionic stationary phase ensures reproductible retention of all types of compounds that have proved difficult to separate on reversed-phase HPLC columns. ZIC®-HILIC is designed for...

  • TSKgel® G2000SWXL HPLC Column phase diol, L x I.D. 30 cm x 7.8 mm, 5 mum particle size

    Widely considered the premium columns for analyzing biopolymers by GFC, TSKgel SWXL columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution. With 5mum particles and 125Å pores, TSKgel G2000SWXL...

  • YMC HPLC ODS-A Reversed-Phase C18 Column

    ODS-A in 120Å pore size is the first choice of many chromatographers for high performance reversed phase separations. It has selectivity for the separation of a wide range of organic compounds found in pharmaceutical, chemical and biotechnology industries. ODS-A is a high coverage, fully endcapped...

  • Lichrospher Reversed-Phase Cartridge Columns

    LiChrospher silica columns are made with spherical “sil-gel” type porous silica particles LiChrospher 60 RP-select B starts with 60Å pore size silica optimized for symmetrical peak shapes of basic compounds. LiChrospher 100Å is offered in both C8 and C18, as well as an...

  • Ascentis® C18 Supelguard(TM) Guard Cartridge

    Legal Information: Supelguard is a trademark of Sigma-Aldrich Co. LLC

  • Superspher Reversed-Phase Columns

    Superspher is a high performance 4 µm silica support. It offers excellent pressure/performance ratio for analysis of complex mixtures requiring high peak capacity. Superspher columns are available as two C18 phases; one endcapped for best peak and one non-endcapped for alternate...

  • ProteCol® Amino Normal Phase HPLC Columns

    Turn imagination into results with the ProteCol™ range of HPLC columns. With Reverse Phase, Normal Phase and Specialty Phases, ProteCol ensures you have the right phase for your separation needs. With the option of combining inert PEEK coated hardware or traditional stainless steel with each...

  • Ascentis® ES-Cyano HPLC Column 5 µm particle size, L × I.D. 15 cm × 4.6 mm

    Features and Benefits Enhanced stability at low pH Operates in reversed-phase, HILIC, and normal phase modes of chromatography Low MS bleed 100% aqueous compatible Available as 3 μm and 5 μm particles Particle composition: Type B silica gel Particle shape: Spherical Extra stable for low pH...

  • YMC HPLC NH2 (Amino) Normal-Phase Column

    YMC-Pack NH2 is a normal-phase separation column utilizing the polarity of primary amino groups. It is also applicable to separations utilizing weak anion exchange. YMC-Pack NH2 is often used for separation of sugars. Application: For Nucleotides Silica gel chemically bonded with aminopropyl group...

  • ZORBAX Method Development Kits

    Agilent Technologies

    Agilent offers a series of kits that allow for fast method development at an attractive price. Each kit contains three columns. To study the effect of a change in selectivity on your separation under a given set of conditions, try either the Eclipse kits for applications in the pH range 2-9 or...

  • PUROSPHER® STAR RP-18 ENDCAPPED (3MYM) HIBAR® RT 50-3 HPLC COLUMN

    PUROSPHER® STAR RP-18 ENDCAPPED (3MYM) HIBAR® RT 50-3 HPLC COLUMN

  • Purospher® STAR RP-18 Endcapped (5 µm) LiChroCART® 4-4

    Purospher® STAR RP-18 endcapped is the best choice for HPLC separation of basic, neutral, metal chelating samples or indeed any other format. Its outstanding performance and stability, allows a maximum flexibility in method development. Robust methods can be developed across the entire pH...

  • ZORBAX Gf-250 and Gf-450gel Filtration Columns

    High efficiency and reproducibility with short analysis time Hydrophilic diol bonded phase for good protein recovery Compatible with organic modifiers and denaturants Wide usable pH range (pH 3-8) ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are ideal for the size separations of...

  • TSKgel® G2000SW HPLC Column phase diol, L × I.D. 30 cm × 7.5 mm, 10 µm particle size

    Synonym: TSKgel® Size Exclusion (SW-Type) HPLC Column Physical form 20S isomer General description As the first generation of silica-based SEC columns, TSKgel G2000SW provides excellent separation of peptides and proteins with molecular weights up to 100,000 Da.TSKgel G2000SW columns start at...

  • Ascentis® Express C18, 5 Micron HPLC Column 5 µm particle size, L × I.D. 3 cm × 2.1 mm

    Ascentis® Express 5 µm C18 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core® particle design. The Fused-Core® particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high...

  • NUCLEOSHELL® HILIC HPLC Columns

    NUCLEOSHELL® core-shell silica phases for HPLC NUCLEOSHELL® Core-shell technology provides Better resolution at lower back pressure and shorter retention time Solid core of silicon dioxide Homogeneous shell of porous silica Highest efficiency compared to traditional totally porous materials...

  • Discovery® RP-AmideC16 HPLC Column

    Discovery® RP-AmideC16 HPLC Column consists of a stationary phase made up of high-purity C16-silica that includes a polar-embedded amide function attached to the silica surface with a propyl group. Application: Discovery® RP-AmideC16 HPLC column may be used for the separation of: •Phenolic...

  • Accucore Polar Selectivity Kits

    Verify optimum selectivity of Thermo Scientific* Accucore HPLC columns for polar analytes. Based on Core Enhanced Technology, Accucore columns provide fast, high-resolution separations - without the elevated backpressures required by sub-2µm particles. Fast separations with excellent resolution and...

  • Discovery® RP-AmideC16 HPLC Column 5 µm particle size, L × I.D. 25 cm × 4.6 mm

    eCl@ss 32110501 Features and Benefits Excellent retention and resolution of polar compounds Unique selectivity compared to C18 Excellent peak shape and efficiency Less hydrophobic than C18 columns Compatible with 100% aqueous mobile phases Legal Information Discovery is a registered trademark of...

  • Lichrospher Si Normal-Phase Cartridge Columns

    Spherical silica for acidic, neutral and basic compounds Great batch-to-batch reproducibility

  • ZORBAX Eclipse XDB Columns

    Agilent Technologies

    Agilent ZORBAX Eclipse XDB columns – C18, C8, Phenyl and CN – provide four bonded phase choices for method development optimization. These columns provide good peak shape over a wide pH range (2-9) for additional method development flexibility with one family of columns. Eclipse XDB...

  • TSKgel® SW Type Guard Column phase diol, L × I.D. 7.5 cm × 7.5 mm, 10 µm particle size

    Synonym: TSKgel® Size Exclusion (SW-Type) HPLC Guard Column General Description Guard Column for 7.5mmID TSKgel G2000SW, G3000SW and G4000SW columns TSKgel Guard Columns are stand-alone and do not require separate holders. Physical Form Shipped in 0.05% NaN3 and 0.1 mol/L Na2SO4 in 0.1 M...

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