Wheat germ (Triticum vulgaris) agglutinin (WGA) is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA). This lectin has proven useful for the purification of insulin receptors and for neuronal tracing studies.
- Inhibiting/Eluting Sugar: 500mM N-acetylglucosamine or 100 mM acetic acid
- Matrix: Cross-linked agarose macro beads, 4%
- Coupling Method: CNBr activation v
- pH stability: 4-9
- Ligand Concentration: 5mg wheat germ lectin/mole of drained gel.
- Supplied in phosphate buffer containing 0.04% sodium azide as a preservative
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