Nucleases for PCR

A 50-kDa protein that specifically inhibits common eukaryotic RNases Only 25 to 40 U is required for a standard transcription reaction. Monitored for endogenous exonuclease and endonuclease activity. Determined to be free of contaminating RNases by incubating RNA transcripts. Applications: RNA...

DNase I, Rnase Free enzyme from bovine pancreas produces no noticeable RNA degradation. Produces no noticeable RNA degradation One unit will completely digest 1 µg of DNA in 10 minutes at 37°C in a 25-µL reaction volume. Incubation of 100-fold molar excess DNase I with RNA for one...