Quality Biological

TBE (TRIS Borate EDTA) is often used in procedures involving nucleic acids, the most common being electrophoresis. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. Applications EDTA is a chelator of divalent cations,...

EDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and reagents,...

Kanamycin sulfate is a water soluble broad spectrum antibiotic obtained from soil bacterium Streptomyces kanamyceticus. It is an aminoglycoside antibiotic effective against Gram-negative and Gram-positive bacteria and mycoplasma. It binds to the 30S ribosomal subunit, inhibits translocation, and...

Amphotericin B is used to prevent the contamination of cell cultures by yeast and multicellular fungi. 100X concentration. Just dilute 1:100 to get the final working concentration of 2.50 µg/mL Antifungal agent Amphotericin B prevents the growth of fungi by causing an increase in fungal plasma...

Tris HCl 2 M pH 6.8 is useful with SDS-PAGE and Electrophoresis. Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). Tris...

Nutritionally rich media designed for growth of pure cultures of recombinant strains. 10 g/L of salt formulation Autoclave sterilized LB Agar, Miller and LB Broth, Miller are based on LB Medium as described by Miller for the growth and maintenance of E. coli strains used in molecular microbiology...

EMEM (Eagle’s Minimal Essential Medium) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations Without L-Glutamine Applications EMEM has a higher amino acid concentration and 2 to 5 times greater vitamin...

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. SDS is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. Popular anionic...

Normal Saline’s isotonic solution is used as a general laboratory diluent. With 9 g/L sodium chloride For non clinical use Applications Normal Saline can be used in the following microbiology applications: solvent, washing agent for cells/bacteria/specimen, buffer in aseptic techniques and plaque...

Applications The FBS in the Cryopreservation Medium improves the post-freezing recovery and survival after cryopreservation, while DMSO preserves the cells during freeze. Cryopreservation Medium with Fetal Bovine Serum (FBS) and Calf Serum is a complete cryopreservation medium with 10% DMSO.

PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications PBS is...

Low-EDTA 1X, pH 8.0 is molecular biology grade TE buffer used to store DNA and RNA. EDTA chelates Mg2 and other divalent metals ions – (inhibits DNAse and RNAse to suppress DNA and RNA degradation). Tris is a buffering agent to keep the solution at a defined pH – The ‘Cl’ or ‘HCl’ indicates the pH...

Formulated as 5X rather than the traditional 2X stocks, SDS Protein Gel Loading Solution 5X enables a larger volume of sample to be loaded per well. 5X concentrated protein gel loading buffer Applications Simply load the gel sample wells and visualize progress of the electrophoresis run by...

Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow. Applications ACK Lysing Buffer is used to lyse red blood cells in samples, such as EDTA-treated whole...

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport...

Cell Freezing Medium A protects cells from damage after freezing and thawing. Applications Cell Freezing Medium A is frequently used in cell biology research for the cryopreservation of cells. Cell Freezing Medium A (2X) works with Cell Freezing Medium B (2X). Recommendation Suspend pelleted cells...

Hanks’ Balanced Salt Solution (HBSS) provides a buffering system to maintain the physiological pH range and osmotic balance of the culture media. HBSS provides cells with a source of water and essential inorganic ions, and carbo Applications HBSS without Ca & Mg can also be useful for diluting...

PCR Water is useful for PCR Assays (i.e rRT-PCR, etc.). DNase, RNase and Protease tested. Free of contaminants of human, mouse and rat DNA. Applications PCR Qualified Water is functionally tested in a PCR reaction. PCR Qualified Water is highly purified water and it is reverse osmosis deionized,...

QBI's Serum Free Cryo Medium is a sterile, 2X concentrated freezing medium containing DMSO  developed for the cryopreservation of cells grown under serum free conditions. Serum Free Cryo Medium is an animal-free, protein-free, chemically defined medium containing DMSO and other...

Calcium Chloride 2 M is useful in the preparation of competent E.coli and transformation of bacteria. Preparation of competent E.coli Transformation of bacteria 2 Molar concentration Filtered through a 0.22 µm membrane Dnase, Rnase, and Protease tested Applications Calcium chloride is a commonly...

Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations Without Phenol Red With Glucose and L-glutamine Applications EMEM (Eagle’s Minimum Essential Medium) has a higher...

Gentamicin is an aminoglycoside complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is used as the sulfate salt. Ready-to-use solution Applications Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition protein synthesis and death...

Can be used for making blocking solution. Blocking solutions decrease the non-specific binding of peptides and proteins to improve assay sensitivity.

Fischer’s medium supports clonal growth of cells – particularly lymphoblasts – from primary explants or cells already in culture. With L-glutamine and Phenol Red Applications Fischer’s medium also supports mammalian cell culture and murine hematopoietic cells. Recommendation Supplement with 1.125...