Boston BioProducts

Components: Citric Acid / Sodium Citrate(0.5 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 4.0 ± 0.15 Storage: 2-8°C

MOPS Buffer (1 M, pH 7.4) - 500ml

HEPES Buffer (1 M, pH 7.6, pH Adjusted with KOH) - 500ml

Components: Bicine(500 mM), Sodium Hydroxide(For pH adjustment) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.5 ± 0.15 Storage: 2-8°C

Components: Tris Base / Tris-HCl(500 mM), Potassium Chloride(54 mM), Sodium Chloride(2.74 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

HEPES Buffer (1 M, pH 7.5, pH Adjusted with KOH) - 500ml

Components: Sodium Phosphate Dibasic(10.1 mM), Potassium Phosphate Dibasic(1.76 mM), Potassium Chloride(2.7 mM), Sodium Chloride(137 mM), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: Room Temp.

Components: Tris Base / Tris-HCl(1M), Sodium Chloride(3 M) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

Components: MES Monohydrate(500 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 5.5 ± 0.15 Storage: 2-8°C

Tris Buffered Saline, TBS (10X, pH 7.4) - 4L

Tris-HCl Buffer (1 M, pH 9.0) - 1L

Tris-EDTA Buffer, TE (pH 7.4) - 500ml

HEPES Buffer (0.5 M, pH 7.0, pH Adjusted with KOH) - 250ml

RIPA Buffer (with EDTA) - 500ml

HEPES Buffer (0.5 M, pH 7.4, pH Adjusted with KOH) - 250ml

Components: Sodium Phosphate Dibasic(202.8 mM), Potassium Phosphate Monobasic(35.2 mM), Potassium Chloride(54 mM), Sodium Chloride(2.74 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

HEPES Buffer (1 M, pH 7.4, pH Adjusted with KOH) - 500ml

Components: Acetic Acid / Sodium Acetate(1 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 4.0 ± 0.15 Storage: Room Temp.

Components: Tris Base / Tris-HCl(250 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.6 ± 0.15 (1X) Storage: Room Temp.

Tris-HCl Buffer (1 M, pH 6.5) - 1L

Components: Sodium Phosphate Dibasic(100 mM), Potassium Phosphate Monobasic(20 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.6 ± 0.15 (1X) Storage: Room Temp.

Components: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(1 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.2 ± 0.15 Storage: Room Temp.

Components: Water() Method: Deionized water (18.2 megohms-cm ± 1) was treated with 0.01% DEPC and incubated at 37°C for 12 hours. DEPC treated water was autoclaved for 2 hours at 15 psi (121°C) on liquid cycle to inactivate DEPC. pH: < 7.0 Storage: Room Temp.

Components: Tris Base / Tris-HCl(50 mM), Sodium Chloride(150 mM), Sodium Deoxycholate(0.5%), Sodium Dodecyl Sulfate (SDS)(0.1%), Nonidet P-40 Substitute(1%) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C