Boston BioProducts

Potassium Phosphate Buffer (0.2 M, pH 7.4) - 1L

Tris-HCl Buffer (1 M, pH 7.4) - 1L

HEPES Buffer (1 M, pH 7.0, pH Adjusted with KOH) - 500ml

Components: HEPES(1 M), Sodium Hydroxide(-), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.0 ± 0.15 Storage: 2-8°C

Components: Tris Base / Tris-HCl(0.5 M), Sodium Chloride(1.5 M), Tween-20(1%), Sodium Azide(0.02%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.6 ± 0.15 (1X) Storage: Room Temp.

HEPES Buffer (0.5 M, pH 7.4) - 250ml

Components: MES Monohydrate(1 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 5.0 ± 0.15 Storage: Room Temp.

HEPES Buffer (1 M, pH 7.6) - 500ml

Tris-HCl Buffer (1 M, pH 7.6) - 1L

Components: Sodium Phosphate Dibasic(101.4 mM), Potassium Phosphate Monobasic(17.6 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

Components: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(500 mM), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 Storage: Room Temp.

Components: Bicine(500 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 Storage: Room Temp.

RIPA Buffer (with Triton X) - 500ml

Tris-HCl Buffer (1 M, pH 6.8) - 1L

Components: Tris Base / Tris-HCl(250 mM), Sodium Chloride(750 mM), Nonidet P-40 Substitute(5%), Sodium Deoxycholate(2.5%), Sodium Dodecyl Sulfate (SDS)(0.5%), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 (1X) Storage:...

Components: Tris Base / Tris-HCl(50 mM), Sodium Chloride(150 mM), Nonidet P-40 Substitute(1%), Sodium Deoxycholate(0.5%), Sodium Dodecyl Sulfate (SDS)(0.1%), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 Storage:...

Components: Bicine(500 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.5 ± 0.15 Storage: 2-8°C

Components: HEPES(0.5 M), Sodium Hydroxide(for pH adjustment), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.0 ± 0.15 Storage: 2-8°C

Tris-HCl Buffer (1 M, pH 8.0) - 1L

Components: Tris Base / Tris-HCl(500 mM), Sodium Chloride(1.5 M) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

EDTA (0.5 M, pH 8.0) - 500ml

Components: Sodium Phosphate Dibasic(100 mM), Sodium Phosphate Monobasic(20 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 (1X) Storage: 2-8°C

Components: HEPES(1 M), Sodium Hydroxide(-), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.5 ± 0.15 Storage: 2-8°C

Components: MES Monohydrate(500 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 6.0 ± 0.15 Storage: 2-8°C