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The Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells provides green/red fluorescent staining of live and dead cells, respectively. The assay employs two probes that detect intracellular esterase activity in live cells and compromised plasma membrane integrity in dead cells. The esterase...
BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an...
This product is prepared by labeling highly cross-adsorbed donkey anti-goat IgG (H+L) with CF®488A dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent...
Near-IR CF® dye Annexin V conjugate is Annexin V labeled with our superior near-IR fluorescent dyes. Annexin V is a 35-36 kDa protein that has a high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the cytoplasmic surface of the cell membrane. In apoptotic cells,...
This product is prepared by labeling highly cross-adsorbed goat anti-human IgG (H+L) with CF®543 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Orange fluorescent CF®543...
CF® Dye TFP Esters are amine-reactive fluorescent dyes for labeling antibodies and other proteins. TFP (tetrafluorophenyl) ester is a more stable alternative to succinimidyl ester (SE or NHS ester). • CF®850 is one of Biotium's next-generation near-infrared dyes with absorption/ emission at 852/870...
This product is prepared by labeling highly cross-adsorbed goat anti-rabbit IgG (H+L) with CF®594 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Deep-red fluorescent...
This product is prepared by labeling highly cross-adsorbed donkey anti-rabbit IgG (H+L) with CF®750 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Near-infrared...
CD4 (clone SK3) is a mouse monoclonal antibody that recognizes the human protein CD4. CD4 is a common marker of Helper T cells, and is a T cell coreceptor. All antibody conjugates have been validated in flow cytometry. Each is supplied at 5 uL/test, with sufficient antibody for either 25 (125uL) or...
The Firefly Luciferase Assay Kit is designed for detection and quantification of firefly luciferase reporter enzyme from cultured cells in a simple, efficient and linear fashion. Kit Components: • 5X Firefly Luciferase Lysis Buffer • D-Luciferin • Firefly Luciferase Assay Buffer (Lyophilized)
NucView® 405 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA)...
Fluorescent conjugates of Alpha-Bungarotoxin bind to the nicotinic acetylcholine receptor with high affinity, and thus can be used to image the distribution of the receptor at the neuromuscular junctions. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness,...
NucView®488 and RedDot™2 Apoptosis/Necrosis Assay Kit contains NucView®488 Caspase-3 Substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity.
This product is prepared by labeling monoclonal mouse anti-biotin with CF®633 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®633 dye has...
Methylene Blue (MB) is a commonly used redox indicator in nucleic acid research. MB-dibenzocyclooctyne (DBCO) allows copper-free bioorthogonal conjugation to spontaneously label molecules containing azide groups.
This product is prepared by labeling highly cross-adsorbed goat anti-human IgG (H+L) with horseradish peroxidase (HRP). To minimize cross-reactivity, the antibody has been adsorbed against bovine, horse, and mouse serum.
5X TBE (Tris-Borate-EDTA) is a concentrated buffer solution in deionized water. TBE is used for polyacrylamide and agarose gel electrophoresis. 1X solution: 89 mM Tris,89 mM boric acid, 2 mM EDTA. Store at room temperature.
This product is prepared by labeling high quality rabbit anti-mouse IgG (H+L) with CF®555 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Orange-red fluorescent CF®555...
Ready-to-use tyramide amplification buffer can be used for tyramide signal amplification with CF® Dye Tyramides or other tyramides without the need to add hydrogen peroxide.
Membrane-impermeant calcium indicator that can be loaded into cells via microinjection or scrape loading. Kd: 250 nM &lamda;Ex/&lamba;Em (no Ca 2 ) = 349/482 nm &lamda;Ex/&lamba;Em (high Ca 2 ) = 331/398 nm ε (349 nm, no Ca 2 ) = 34,000 M...
ViaFluor® SE Cell Proliferation Dyes are fluorescent tracers for tracking cell division by flow cytometry; ViaFluor® 405 SE Cell Proliferation Dye is an improved replacement for the classical proliferation dye CFSE. It can be detected in the Pacific Blue channel with violet laser excitation.
This product is prepared by labeling monoclonal mouse anti-biotin with CF®770 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Near-infrared fluorescent CF®770 dye has...
This product is prepared by labeling highly cross-adsorbed donkey anti-mouse IgG (H+L) with CF®647 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent...
Mix-n-Stain™ DNP antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your antibody with DNP in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. Choose from kit sizes to label...