Biotium

Aquaphile coelenterazine native is a bioluminescence substrate in a special formulation format that is readily dissolved in water or PBS buffer for in vivo use. Yellow solid soluble in water or PBS buffer Store at -20°C and protect from light C₂₆H₂₁N₃O₃...

Rose-Beta;-D-Gal (6-Chloro-3-indoxyl-beta-D-galactopyranoside) is a chromogenic substrate for β-galactosidase. The product is similar to X-gal, but generates an intense pink colored precipitate (λ_max ~ 540 nm) on enzymatic hydrolysis. White solid soluble in DMSO Store at 4°C...

This product is prepared by labeling highly cross-adsorbed goat anti-chicken IgY (H+L) with CF®568 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF®568...

MemBrite Fix-ST 650/665 is a far-red fluorescent dye that covalently labels the surface of live cells. MemBrite Fix-ST dyes are well-retained after fixation and permeabilization, and are compatible with STORM super-resolution imaging.

1,6-Diphenyl-1,3,5-hexatriene (DPH, excitation/emission=350 nm/420 nm) is almost non-fluorescent in water, but it exhibits a strong increase in fluorescence after intercalation into membranes and can be used as a probe for viscosity, polarity and lipid order. DPH has been used for the high-content...

This product is prepared by labeling high quality rabbit anti-human IgG (H+L) with CF®633 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®633 dye...

This product is prepared by labeling highly cross-adsorbed goat anti-rat IgG (H+L) with CF®660C dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent...

CF®DI dye hydrazide could be used to react with aldehyde or ketone groups from protein carbonylation. CF®DI dyes are designed to match for charge and molecular weight for 2D DIGE platform. • Orange fluorescent CF®555DI has excitation/emission at 547/572 nm. • Far-red fluorescent CF®647DI has...

This product is prepared by labeling highly cross-adsorbed donkey anti-rat IgG (H+L) with CF®660R dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent...

Goat anti-mouse IgG2b isotype-specific secondary antibody labeled with CF®770 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Near-infrared fluorescent CF®770 dye has...

The PMA Real-Time PCR Bacterial Viability Kit for Legionella pneumophila (mip gene) allows for specific detection of live Legionella

MTS-CR110-X (MTS-5-(and-6)-carboxyrhodamine 110-X, mixed isomers) is a green fluorescent thiol-reactive dye. It is similar to MTS-CR110, except that the former has a X spacer molecule between the dye and the MTS reactive group. The linker molecule may enhance fluorescence when the dye is attached to...

This product is prepared by labeling highly cross-adsorbed donkey anti-human IgG (H+L) with CF®633 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent...

Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic...

The AccuBlue® Broad Range dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The solution provides a linear detection range from 2 - 2000 ng and is compatible with fluorescence microplate readers that can...

This product is prepared by labeling high quality rabbit anti-goat IgG (H+L) with CF®640R dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®640R dye...

For ease of use and safer handling, we offer both Hoechst 33258 and Hoechst 33342 dissolved in H2O at 10 mg/mL. Hoechst dyes are cell membrane-permeant, minor groove binding blue fluorescent DNA stains.

NucSpot® Live 488 Live Cell Nuclear Stain is a green fluorescent (excitation/emission 500/515 nm) DNA dye that stains nuclei in live or fixed cells. It has excellent specificity for DNA without the need for a wash step, with low toxicity for live cell imaging. Supplied as a 1000X stock solution in...

Biotium's goat anti-human IgG, Fc gamma specific antibody is labeled with CF®488A dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF®488A dye has...

2X Forget-Me-Not™ Universal qPCR Probe Master Mix is a high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping. • Suitable for all fluorescent probe types, including TaqMan®, BHQ®, and molecular beacons • Sensitive, robust, and reproducible...

Indo-1, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate cell loading. Indo-1/AM itself does not bind Ca 2 , but it is...

EverBrite™ Hardset with NucSpot® 640 is a hardset antifade mounting medium with Biotium's unique far-red nuclear dye (excitation/emission 649/668 nm with DNA), for permanent mounting and counterstaining of immunofluorescence. Note: NucSpot® 640 has dim blue fluorescence, and may not be compatible...

The Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells provides green/red fluorescent staining of live and dead cells, respectively. The assay employs two probes that detect intracellular esterase activity in live cells and compromised plasma membrane integrity in dead cells. The esterase...

BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an...