Features & Benefits:
Unbiased Lysis: Efficient and unbiased lysis of microbes including gram positive/negative bacteria, fungi, protozoans, and viruses from any sample including feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.
Ultra-Pure: High-quality DNA/RNA (including mall/microRNAs) are inhibitor-free and ready for RT/qPCR and microbiome measurements using Next-Gen sequencing.
High Sensitivity: Increased detection limit of very low abundance organisms.
The ZymoBIOMICS MagBead DNA/RNA kit provides a high-throughput, magnetic bead-based purification of both high-quality DNA and total RNA (including small/microRNAs) from the same starting sample. The provided DNA/RNA Shield inactivates infectious agents and is ideal for sample storage at ambient temperatures. The extraction method utilizes magnetic beads for DNA/RNA extraction without the use of phenol and is eluted into ≥50 µl of ZymoBIOMICS DNase/RNase-Free Water. DNA/RNA is ready for any downstream application including Next-Gen Sequencing, RT-qPCR, etc.
Binding Capacity 15 µg DNA/RNA per 30 µl ZymoBIOMICS MagBinding Beads
Equipment Needed Magnetic stand or separator, heat block, liquid handler or robotic sample processer (user provided)
Purity High-quality RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc
Recommended Materials (sold separately) – 96-well Collection Plate (C2002; capacity is up to 1.2 ml/well), 96-Well Block (P1001; capacity is up to 2 ml/well), 96-well Elution Plate (C2003), Cover Foil (C2007), ZR-96 MagStand (P1005)
Sample Preservation DNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures
Sample types Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from ≤ 50 mg of mammalian feces, ≤ 50 mg soil, and 5-20 mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs
Size Limits Capable of recovering DNA and total RNA ≥ 17 nucleotides
Storage DNA and RNA eluted with ZymoBIOMICS DNase/RNase-Free Water (provided)can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage