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Zymo Research Corporation

ZymoBIOMICS HMW DNA Standard

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  • Long-Read Sequencing Ready: Ideal to benchmark 3rd generation sequencing and metagenomic measurements (e.g. Oxford Nanopore & PacBio)
  • High Molecular Weight: DNA standard is >50 kb in size
  • Accurate Composition: Composition is cross-validated with multiple types of measurements

ZymoBIOMICS HMW DNA Standard is a mixture of high molecular weight genomic DNA isolated from pure cultures of seven bacterial and one fungal strains. It has an accurately defined composition, negligible impurities (<0.01%) and contains genomes of a wide range of GC content (15%-85%). This can be used to assess and benchmark the performance of long read/3rd generation sequencing for microbiomics and metagenomics analysis and can also be used as a routine quality control.

Theoretical Composition Based on Genomic DNA: Pseudomonas aeruginosa - 14%, Escherichia coli - 14%, Salmonella enterica - 14%, Enterococcus faecalis - 14%, Staphylococcus aureus - 14%, Listeria monocytogenes - 14%, Bacillus subtilis - 14%, Saccharomyces cerevisiae - 2%.

Impurity Level<0.01% foreign microbial DNA
Sample Storage-20°C
SourceSeven bacteria (3 gram-negative and 4 gram-positive) and 1 yeast.
Storage Solution10 mM Tris-HCl and 0.1 mM EDTA, pH 8.0

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Product Detail
Thomas No.
CHM00B731
Mfr. No.
D6322
Description
ZymoBIOMICS HMW DNA Standard
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