ZymoBIOMICS 96 MagBead DNA Kit – Dx (Lysis Tubes)

Applicable For

All sensitive downstream applications such as qPCR and Next-Generation Sequencing.

Elution Volume

≥ 37.5 µl DNA Elution Buffer or DNase free water

Equipment

Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block

Processing Volume

Fecal ≤100 mg, Soil ≤ 200 mg, Cells ≤ 20 mg (Approximately equal to 2x10⁸ bacterial, 2x10⁷ yeast, or 2x10⁶ mammalian cells)

Purity

High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing

Sample Source

Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water.

Sample Storage

Eluted DNA should be stored at ≤ -20°C.

Size Range

Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.

Type

Total DNA

Yield

Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum)