Synonym: 5,6-Epoxy-4,27-dihydroxy-1-oxowitha-2,24-dienolide, Withaferine A
CAS Number: 5119-48-2
Empirical Formula (Hill Notation): C28H38O6
Molecular Weight: 470.60
MDL Number: MFCD10687098
Biochem/physiol Actions
Steroidal lactone that exhibits cytotoxicity towards tumor cells. Protective effects attributed to anti-lipid peroxidative, antioxidant and detoxifying functionality.
Withaferin A is a steroidal lactone that is isolated from the plant Withania somnifera. It has potent anti-inflammatory properties as it inhibits the activation of NF-κ B signaling pathway.2 The anti-tumor activity of Withaferin A is due to its ability to alter cytoskeletal architecture by binding annexin II and disrupting F actin cross-links.3 Withaferin A also inhibits angiogenesis by binding to vimentin and F-actin.4
Preparation Note
Withaferin A yields a clear, colorless solution in methanol at 1 mg/ml.
Application
Withaferin A was used to treat HEK293 cells to study its effect on cystic fibrosis inflammation.1