The measurement of TG levels, in conjunction with other lipid assays, are useful in the diagnosis of primary and secondary hyperlipoproteinemia, dyslipidemia, and triglyceridemia. Cayman’s TG Assay provides a simple, reproducible, and sensitive tool for assaying TGs in plasma, serum, cell lysate, and tissue homogenate samples. The assay is initiated with the enzymatic hydrolysis of the TGs by lipase to produce glycerol and free fatty acids. The glycerol released is subsequently measured by a coupled enzymatic reaction system with a colorimetric readout at 540 nm.