Synonyms: Tris hydrochloride solution
Application: The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Other Notes: Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.
Legal Information: Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Concentration: 1 M
Grade: for molecular biology
Impurities: DNase, RNase, NICKase and protease, none detected
pH: 7.4
Product Line: BioReagent
Quality Level: GMP
Sterility: 0.2 μm filtered