ExtrAvidin(R)-FITC, buffered aqueous solution

MDL No.: MFCD00163600

Storage: -20C

UNSPSC Code: 12352203

Application: ExtrAvidin(R)-FITC is suitable for the following applications: . Immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a concentration of 20μg/mL using human tissues.. Indirect immunofluorescence at a dilution of 1:200. Immunofluorescence.

Biochem/physiol Actions: Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin is prepared from egg white avidin. It is a modified form of affinity purified avidin and combines high specific activity of avidin with the low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin has been conjugated to fluorescein isothiocyanate (FITC) and is used for various techniques.

Physical form: Solution in 0.01 M phosphate buffered saline, containing 10% (v/v) 0.5 M carbonate buffer, pH 9.5, and 15 mM sodium azide

Preparation Note: Affinity purified protein