Synonyms: Anti Rabbit; Anti Rabbit Antibody; Anti Rabbit Igg; Anti-Rabbit Igg; Goat Anti Rabbit IgG Antibody - Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in goat; Goat Anti Rabbit Igg
MDL No.: MFCD00162788
Storage: -20C
UNSPSC Code: 12352203
General description: IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-rabbit IgG-peroxidase conjugate associates with all rabbit Igs. The conjugate shows no reaction with human IgG by immunoelectrophoresis (IEP). Furthermore, the antibody is adsorbed with human IgG to reduce background staining with human samples.
General description: Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE. It has only one IgG variant with one interheavy disulfide bridge. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Application: Goat anti-rabbit IgG conjugated to peroxidase can be used for direct ELISA and immunohistochemistry.
Application: Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.
Application: Peroxidase-conjugated goat anti-rabbit IgG antibody was used for western blot analysis of Cos 7 cell lysates at a 1:2000 dilution and yeast lysates following protein carbonylation assays at a dilution of 1:5000. Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in goat has been used as a secondary antibody during immunofluorescence staining.
Application: Anti-Rabbit IgG (whole molecule)-Peroxidase antibody has been used in western blotting , immunofluorescence staining , immunochemistry and immunoprecipitations.
Immunogen: purified rabbit IgG
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal
Preparation Note: Prepared by two-step glutaraldehyde method.
RIDADR: NONH for all modes of transport
WGK Germany: 2