MDL No.: MFCD00162699
Storage: -20C
UNSPSC Code: 12352203
General description: Immunoglobulins are proteins produced by B cells in response to antigen and regulate response to bacteria, and viruses. IgG is known to regulate complement fixation and placental transport. IgA has a crucial role in mucosal immunity as it restricts pathogens from entering the mucosal membrane. IgM is the largest antibody having a pentameric structure which modulates polyreactivity and removes apoptotic cells,,. Goat anti-mouse polyvalent immunoglobulins (G,A,M)-peroxidase antibody binds to mouse IgG, IgA and IgM.
Application: The detection of antibodies in sera of T. gondii-infected mice was done by ELISA using peroxidase-conjugated anti-mouse polyvalent immunoglobulin antibody as the secondary. Relative binding affinities for specific Mabs were determined by an inhibition ELISA using peroxidase-conjugated goat anti-mouse polyvalent antibody diluted 1:10,000 in PBST and incubated for 1 hour at 21-23C.
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 0.01% thimerosal
Preparation Note: Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
RIDADR: NONH for all modes of transport
WGK Germany: 2