Anti-Lamin A (C-terminal) antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyms: Anti-LAMA; Anti-LMN1; Anti-LMNA; Anti-Lamin A/C

MDL No.: MFCD01633588

Storage: -20C

UNSPSC Code: 12352203

General description: Lamin A is a structural protein found in the nuclear lamina . This protein is cut into a 47kDa fragment that facilitates chromatin condensation and nuclear degradation during cell death Anti-Lamin A (C-terminal) antibody is specific for lamin A in mice, rats and humans. The antibody does not react with lamin C. Lamin A detection by immunoblotting is specifically inhibited with the immunizing peptide.

Application: Anti-Lamin A (C-terminal) antibody is suitable for use in western blot (0.1-0.2 mug/mL) using HeLa nuclear extract. This antibody can also be used in indirect immunofluorescence (1-2 mug/mL) using HeLa cells, rat NRK and mouse 3T3 cells. Additionally, anti-Lamin A (C-terminal) antibodies are suitable for use in immunoblotting (approx. 70 kDa). Cleaved fragments of lamin A may form additional bands at 45-50 kDa.

Immunogen: synthetic peptide corresponding to amino acids 598-611 of human lamin A with a C-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by one amino acid in rat, three amino acids in mouse, and by a gap of one amino acid in both rat and mouse lamin A.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.