MDL No.: MFCD00162453
Storage: 2-8C
UNSPSC Code: 12352203
General description: Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains, with molecular weight of 50kDa and 25kDa respectively. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Goat anti-human IgG binds to human IgG.
Application: Serum human IgG levels were analyzed by solid phase ELISA assays using alkaline phosphatase-conjugated goat anti-human IgG antibody incubated for 1 hour at 37C. Assays was developed using 0.1% p-nitrophenyl phosphate (Sigma) as the substrate. Antibodies to the Seoul (strain 80/39) virus was detected in human sera by western blot analysis using alkaline phosphatase-conjugated goat anti-human IgG antibody at a 1:1000 dilution. Blots were developed using nitroblue tetrazolium and 5-bromo-4-chloro-3-indoyl-phosphate substrate (Sigma).
Application: Goat anti-human IgG can also be used for dot blot (1:30,000) and immunohistochemical (1:50) applications.
Immunogen: IgG purified from human serum
Physical form: Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide
RIDADR: NONH for all modes of transport
WGK Germany: 2