MDL No.: MFCD00162443
Storage: 2-8C
UNSPSC Code: 12352203
General description: Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Goat anti-human IgG (gamma-chain specific)-alkaline phosphatase antibody is specific for human IgG when tested against human IgA, IgG, IgM, Bence Jones Kappa, and Lambda myeloma proteins.
Application: Proteins harvested from E. coli cultures were run on SDS-page gel for western blot analysis using alkaline phosphatase conjugated goat anti-human IgG (chain specific) at a concentration of 1:10000 for the secondary. IgG was detected in human serum by Elisa using alkaline phosphatase conjugated goat anti-human IgG (gamma-chain specific) diluted 1:5000 in 0.25% BSA/PBS. Assay was developed using p-nitrophenyl phosphate substrate (1mg/ml Sigma).
Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Enzyme-linked immunosorbent assay (1 paper)
Application: Goat anti-human IgG (gamma-chain specific)-alkaline phosphatase antibody can be used for ELISA applications using serum samples from allergic and borderline hypertensive patients.
Immunogen: purified human IgG
Physical form: Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 15 mM sodium azide and 50% glycerol
RIDADR: NONH for all modes of transport
WGK Germany: 2