Synonyms: 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside; 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside; bromo-4-chloro-3-indolyl-β-d-galactopyranoside, 5-; 5-Bromo-4-chloro-3-indolyl β-D-galactoside; BCIG; X-Gal
Empirical Formula (Hill Notation): C14H15BrClNO6
Formula Weight: 408.63
Linear Formula: C14H15BrClNO6
MDL Number: MFCD00005666
Storage: 2-8°C
Beilstein Registry Number: 1402009
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
General description:
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. It is soluble in dimethylformamide and methanol. The product is not soluble in water.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Chromatographically homogeneous
Physical form:
Crystals, white powder
Preparation Note:
Working solution: Stock solution:• 20 mg/ml in dimethyl formamide (DMF), clear, colorless solution
• A590 nm: 0.01
Storage conditions (working solution): X-gal in solution (i.e., dissolved in DMF 2% (w/v)) is stable for several weeks when stored in the dark at 2 to 8 °C; when the solution is stored at -15 to -25 °C, it is stable for one year.