Synonyms: Substrate of protease; casein, resorufin-labeled; protease substrate, universal
Storage: −20°C
UNSPSC Code: 12352204
RIDADR: NONH for all modes of transport
Application:
Principle
By treatment with proteases, resorufin-labeled peptides are released from resorufin-labeled casein. They cannot be precipitated by trichloroacetic acid. The concentration of these resorufin-labeled peptides in the supernatant is equivalent to the proteolytic activity present.
General description:
The Universal Protease Substrate provides rapid and highly sensitive detection of trace protease activity, or determination of the effectiveness of protease inhibition. Protease activity releases resorufin-labeled peptides from the patented substrate, resorufin-labeled casein. Resorufin is N-(resorufin-4-carbonyl)-piperidine-4-carbonic acid).The concentration of these labeled peptides in the supernatant is directly related to the proteolytic activity present.
• Conveniently detect nanogram quantities of proteolytic activity in less than one hour.
• Perform highly sensitive protease detection in a homogeneous assay.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Function test: performance-controlled in the assay for proteases.
Preparation Note:
Working concentration: Approximately 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
An aqueous solution is stable for several months at -15 to -25° C and for 2 to 3 days at 2 to 8° C. It is recommended to store aqueous solutions in aliquots at -15 to -25° C. Repeated freezing and thawing is possible. At 15 to 25 °C the product is rapidly hydrolyzed in solution.