Purity: 95% (SDS-PAGE)
Specific Activity: >2 x 10^7 U/mg
Storage: −20°C
UNSPSC Code: 12352207
RIDADR: NONH for all modes of transport
Application:
Interferon-γ, human (hIFN-γ), can be used to investigate IFN-γ activities in human cell systems.
Biochem/physiol Actions:
A broad range of biological activities has been attributed to IFN-γ (e.g., the establishment of the antiviral state, immunoregulatory functions, antiproliferative effects and inhibition of cell growth). The anti-proliferative effects of IFN-γ are superior to those of either IFN-α or IFN-β. Growth inhibition is dependent on cell type, dose, and length of exposure. One of IFN-γ’s primary functions might be as an immunoregulatory agent: IFN-γ induces MHC antigens on many cells, Fc-receptors on monocytes and macrophages, IL-2 receptors on T-cells, enhances activity of macrophages, polymorphonuclear leukocytes, T-lymphocytes, and NK-cells (MAF), and is also involved in the regulation of B-cells.
General description:
IFN-γ is produced by T-lymphocytes stimulated by antigen or by T-cell mitogens. Under nondenaturating conditions its molecular weight values range from 32,000 to 73,000 indicating that recombinant human IFN-γ exists as a dimer or higher oligomers.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
The raw material used for this preparation was tested for HBs antigen and for the presence of antibodies to HIV-1, HIV-2, and HCV found to be negative.
Endotoxin level: <0.1EU/μg (LAL-test), <10EU/ml (LAL-test)
Note: 1EU corresponds to 0.1ng
Physical form:
100,000U/ml or 1,000,000U/ml of Human IFN-γ in 0.1M phosphate buffer saline (PBS) (pH 7.0), 2.5% sucrose (w/v), and 2.5% human serum albumin (HSA) (w/v), filtered through a 0.2μm pore size membrane.
Preparation Note:
Working solution: Solvent is recommended in double-distilled water.
Storage conditions (working solution): Stability in culture: Activity is present for at least 18 hours in culture medium.
Sequence:
Chain Length 143 AA
Specificity:
Human IFN-γ is effective on human cells, but not on mouse or rat cells.