Purity: >98% (Transferrin, SDS-PAGE); >99% (Insulin, HPLC); >99% (Sodium selenite)
Storage: 2-8°C
UNSPSC Code: 12352207
RIDADR: NONH for all modes of transport
Application:
The Insulin-Transferrin-Sodium Selenite Supplement is used in the cell culture media as a basal growth-factor supplement to which other cell type-specific nutrients and growth factors are added.
General description:
The Insulin-Transferrin-Sodium Selenite Supplement contains the most essential growth-promoting components of serum-free culture media in an optimized ratio.
Source: Insulin- bovine pancreas; transferrin- human serum; Sodium selenite- synthetic
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Preparation Note:
Working concentration: Insulin, 5 μg/ml, transferrin, 5 μg/ml, sodium selenite, 5 ng/ml (3.0 x 10-8 M)
Storage conditions (working solution): -15 to -25 °C.
Prepare appropriate aliquots and avoid repeated freezing and thawing.
Reconstitution:
The insulin-transferrin-sodium selenite supplement should be reconstituted in 5 ml or 25 ml sterile double-dist. water (1000 x concentrated stock solution).
Further dilution with culture medium.