Storage: −20°C
UNSPSC Code: 41106000
RIDADR: NONH for all modes of transport
Application:
• RNA labeling with Fluorescein-12-UTP by in vitro transcription with SP6, T7, and T3 RNA polymerases. Fluorescein-labeled RNA is used in a variety of hybridization techniques:
• In situ hybridization to tissues
• Southern blots
• Northern blots
• Plaque or colony lifts
• RNase protection experiments
General description:
Convenient nucleotide mixture for the labeling of RNA with Fluorescein-12-UTP.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
The mix is function tested in combination with the DIG RNA Labeling Kit.
Preparation Note:
10x concentrated NTP labeling mixture: 10mM ATP, 10mM CTP, 10mM GTP, 6.5mM UTP, 3.5mM Fluorescein-12-UTP, pH 7.5 (+20°C).
Specificity:
Heat inactivation: Stop the reaction by adding 2μl 0.2M EDTA (pH 8.0).
Principle:
Fluorescein-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Fluorescein-12-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The Fluorescein RNA Labeling Mix is specifically designed for use with the RNA polymerases SP6, T7, and T3, which are supplied with an optimized transcription buffer.