Storage: −20°C
UNSPSC Code: 41105335
RIDADR: NONH for all modes of transport
Application:
DNA Molecular Weight Marker VII, DIG-labeled, is used as a size standard in Southern blot analysis when the DIG System for Nucleic Acid Labeling and Detection is used.
General description:
Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
DNA Molecular Weight Marker VII, DIG-labeled is a ready-to-use solution in 10mM Tris-HCl, 1mM EDTA, pH 8.0. The fragment mixture is prepared by cleavage of SPP1 DNA with EcoRI. the size ranges from 0.081 to 8.57kbp.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Sequence:
The mixture contains 17 fragments wit the following base pair lengths: 81, 359, 492, 710, 718, 992, 1164, 1482, 1515, 1882, 1953, 2799, 3639, 4899, 6106, 7427, and 8576 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.