Isolate all sizes of circulating DNA from plasma and serum samples
Isolate viral and bacterial DNA
Versatile plasma and serum input volumes
Concentrate circulating DNA into a flexible elution volume
Isolate inhibitor-free cell-free circulating DNA
Compatible with Streck Cell-Free DNA BCT® Tubes
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits provide a fast, reliable and convenient spin column method for the isolation of high quality, high purity and inhibitor-free cell-free circulating DNA (cfc-DNA) from plasma/serum sample volumes. The purified plasma/serum cfc-DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.
Background
Plasma/Serum cell-free circulating DNA (cfc-DNA) has the potential to provide biomarkers for certain cancers and disease states as well as fetal DNA in maternal blood. Currently, significant advancements are being made in utilizing cfc-DNA as biomarkers for the early diagnosis, prognosis and monitoring of therapy for several cancer types and autoimmune diseases. Cell-free mitochondrial DNA (cfmtDNA) is also under investigation for its clinical significance. This cfc-DNA is usually present as short fragments of less than 1000 bp. In addition, cell-free fetal DNA has been widely used as a non-invasive method for prenatal diagnosis including early identification of fetal sex, genetic studies for families at high risk for inherited genetic disorders, screening for Rhesus factor, screening for aneuploidy and identification of preeclampsia.
Plasma/Serum Cell-Free Circulating DNA Purification Micro Kit
Ideal for handling initial plasma/serum volumes ranging from 10 µL up to 200 µL. The kit is designed to isolate all sizes of cfc-DNA from either fresh or frozen plasma/serum samples and the purified DNA is eluted into a flexible elution volume ranging from 25 µL to 50 µL.
Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit
Ideal for handling initial plasma/serum volumes ranging from 200 µL up to 500 µL. The kit is designed to isolate all sizes of cfc-DNA from either fresh or frozen plasma/serum samples and the purified DNA is eluted into a flexible elution volume ranging from 25 µL to 50 µL.
Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit
Ideal for handling initial plasma/serum volumes ranging from 1 mL - 4 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL
Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit
Ideal for handling initial plasma/serum volumes ranging from 5 mL- 10 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL