KE10083 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse TSLP ELISA kit is to be used to detect and quantify protein levels of endogenous mouse TSLP. The assay recognizes mouse TSLP. An antibody specific for mouse TSLP has been pre-coated onto the microwells. The mouse TSLP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse TSLP is added to detect the captured mouse TSLP protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.