The Na+/K+ ATPase (NKA) enzyme contains a, ß and ? subunits. This enzyme is widely expressed. ATPase Na+/K+ transporting subunit a 1 (ATP1A1) is a principal catalytic subunit of NKA. It has a molecular mass of about 110kDa with 10 transmembrane segments. a-subunit has four different isoforms with tissue specific expression. a1 is primarily expressed in various tissues, including kidney, nerves and lung. While a2 is expressed in skeletal muscle and heart, a3 is found in the brain and a4 is apparently localized to testis and specifically to spermatozoa. ATP1A1 is encoded by the gene mapped to human chromosome 1p13-p11.
MDL Number: MFCD02096998
Storage: -20C
Application: Monoclonal Anti- Na+/K+ ATPase ( a Subunit) antibody produced in mouse has been used in flow cytometry.
Biochem Physiol Actions: The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.