Monoclonal ANTI-FLAG(R) M2 antibody produced in mouse, 1 mg/mL, clone M2, affinity isolated antibody (Affinity tag purification), buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Synonyms: flag antibody; Anti-ddddk; Anti-dykddddk

Storage: -20C

UNSPSC Code: 41106000

Features and Benefits: Antibody BioguaranteeEvaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.Reduced non-specific binding in western blots.

General description: The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Application: For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.Western Blotting and EIABrowse additional application references in our FLAG(R) Literature portal.

Immunogen: FLAG; peptide sequence DYKDDDDK

Physical form: Contains 50% glycerol in phosphate buffered saline.

Specificity: Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Preparation Note: Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk