Monoclonal Anti-Actin, a-Smooth Muscle-HRP (mouse IgG2a isotype) is derived from the hybridoma 1A4 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a synthetic peptide corresponding to a sequence of a-smooth muscle actin, conjugated to KLH. Actin is a highly conserved protein, expressed as six isoforms and represents differentiation markers of muscle tissues.
Synonyms: AAT6; ACTSA; MYMY5; actin; alpha 2; aorta; smooth muscle
Storage: -20C
Application: Monoclonal Anti-Actin, α-Smooth Muscle - Peroxidase antibody has been used in:• direct-immunoblotting• direct-immunohistochemistry• direct- enzyme linked immunosorbent assay (ELISA)• direct staining of tissues and cells• characterization of stromal cell heterogeneity in various organs and distinguish smooth muscle cells from fibroblasts in mixed cultures.
Biochem Physiol Actions: Actin and Myosin are two major cytoskeletal proteins implicated in cell motility, both constitutively expressed in many cells types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. It has been shown that relative proportions of actin isoforms are diverse in smooth muscles of different organs and change within the same population of smooth muscle cells during development, pathological situations and different culture conditions.