Cell migration occurs in a variety of biological processes including wound healing, embryonic development, and immune response. Because of the importance of cell migration in angiogenesis, oncology, neurology, and especially immunology, there is an increased interest in understanding the underlying biological mechanisms at a single cell level. Traditional multiwell (Boyden chamber) migration assays can have limitations, such as: (1) gradients are not linear, well-established, or controlled, (2) quantification of migratory cells is based on an endpoint measurement rather than a dynamic determination, and (3) imaging of the cells while they are migrating is currently not possible.The Millicell µ-Migration Assay Kit overcomes these limitations.Its microfluidic, low-volume technology promotes a stable, diffusion-generated concentration gradient that is consistently linear and lasts for more than 48 hours. Designed for video microscopy assays, the µ-Migration Slide is made from a plastic with high optical qualities similar to those of glass. At specific time intervals, images of the observation area can be acquired, allowing real-time monitoring and quantitative measurements of cell migration.Benefits
•Live cell tracking of single cell or cell population migration
•Establishes a stable and linear concentration gradient that lasts >= 48 hours
•Helps distinguish chemotaxis from random movement
•Allows for multiparametric analysis, including cell directionality and velocity
•Enhanced optical imaging of slow- and fast-migrating cells
•Analyze 3 chemoattractants in parallel for increased throughput and flexibility
•No need to spend time or money on optimization
•Ready to use, no assembly needed How the Kit WorksFor detailed videos and instructions on the use of the µ-migration kit, please visit: Millicell µ-Migration Assay KitFor instructions on how to access and use the free Image-J plug-in software to analyze the data and images collected using the µ-Migration Assay Kit, please visit: Millicell µ-Migration Assay Kit.
Legal Information: Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany