M9 Minimal Media Agar Plates are commonly used for the cultivation of bacteria. M9 minimal medium is comprised of M9 minimal salts base formulation that can be supplemented with various metabolites. It is useful for studying metabolism as you can control the nutrients available.
Commonly used chemically defined minimal media include MOPS Minimal Media and M9 Minimal Media. For research that requires low availability of iron or other metals, MOPS Minimal Media is the appropriate choice. M9 contains high levels of phosphate, which chelates metals that could contaminate the media. Also, certain bacterial species grow better in one medium compared to the other; for example, the preferred choice for Pseudomonas aeruginosa is MOPS Minimal Media.
Typically, 100 mm round plates are for growth/selection, while 150 mm round plates are for screening.
Contains 1X M9 salts, 1.0% glucose, 1.0 mM thiamine, 0.2 mM magnesium sulfate, 0.1 mM calcium chloride, and 1.5% agar.