Background
Latent membrane protein 1 acts as a CD40 functional homolog to prevent apoptosis of infected B-lymphocytes and drive their proliferation. It functions as a constitutively active tumor necrosis factor receptor that induces the activation of several signaling pathways, including those of the NF-kappa-B family. LMP1 signaling leads to up-regulation of antiapoptotic proteins and provide growth signals in latently infected cells. It interacts with host UBE2I and subsequently affects the sumoylation state of several cellular proteins. For example, LMP1 induces the sumoylation of host IRF7 thereby limiting its transcriptional activity and modulating the activation of innate immune responses.
Application Note
Anti-LMP-1 (Epstein-Barr Virus) (MOUSE) Monoclonal Antibody was tested in IHC-P with positive control infected lymphoblastoma, Hodgkin’s lymphoma tissues. Cellular Localization: Cytoplasmic. The user is advised to validate the use of the products with their tissue specimens prepared and handled in accordance with their laboratory practices. This antibody stains strongly with EBV positive lymphoblastoid cell lines and EBV infected B cell immunoblasts in infectious mononucleosis. Consult references (Kiernan, 1981: Sheehan & Hrapchak, 1980) for further details on specimen preparation.