LB Miller broth is another variation of the standard Luria-Bertani (LB) broth, used primarily for growing bacterial cultures in microbiology and molecular biology. LB Miller broth has some differences in its formulation compared to other LB media, which can affect bacterial growth and experimental outcomes.
10g Tryptone, 10g Yeast Extract, 5g NaCl, pH Adjusted to 7.0 +/- 0.1
Antibodies/Concentrations to Choose From:
• 25µg/mL Kanamycin
• 50µg/mL Kanamycin
• Animal-Free Soytone and Kanamycin-25µg/mL
• Animal-Free Soytone and Kanamycin-50µg/mL
• 50µg/mL Ampicillin
• 100µg/mL Ampicillin
• 50µg/mL Carbenicillin
• 100µg/mL Carbenicillin
• Animal-Free Soytone and Kanamycin-50µg/mL
Uses
Bacterial Growth: It supports the growth of a variety of bacteria, including E. coli and other commonly used strains.
Recombinant DNA Technology: Frequently used in cloning experiments and protein expression studies where high bacterial yields are desired.
General Research: Useful in various microbiological applications, including antibiotic resistance studies and metabolic assays.