Storage: -196C
UNSPSC Code: 12352200
Cell Line Origin: Distal oesophageal adenocarcinoma,
Culture Medium: RPMI-1640:Hams F12 1:1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
DNA Profile: STR-PCR Data: Amelogenin: X CSF1PO: 11 D13S317: 11,12 D16S539: 11 D5S818: 10,13 D7S820: 11,12 THO1: 6 TPOX: 8 vWA: 14
Subculture Routine: Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. There is a requirement to change the culture medium every 2-3 days.
Other Notes: Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
RIDADR: NONH for all modes of transport